PMID: 9420132Jan 7, 1998Paper

Specific humoral immune responses in 12 cases of food sensitization to sesame seed

Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology
M N Kolopp-SardaG C Faure

Abstract

Hypersensitivity to sesame seeds is becoming increasingly frequent, probably owing to the larger use of this compound in international food. This study investigated serum responses of 12 sesame sensitized patients (seven with food allergy, five with food sensitization), to a sesame protein extract, and attempted at identifying sesame major antigens. Sesame protein extracts were prepared from black, white and brown sesame seeds. Electrophoretic analysis showed similar protein patterns in the three extracts, and proper preservation of the proteins integrity. The brown sesame extract was used to set-up an ELISA assay and measure serum levels of antisesame IgG, IgA, IgM and IgE in 12 samples from sesame-sensitized individuals and six controls. It also allowed to perform western blot analyses in order to investigate the molecular weight of sesame proteins recognized by IgG, IgA and IgE. Nineteen protein bands were observed upon polyacrylamide gel electrophoresis of the sesame protein extracts. Using this whole extract in ELISA, significant antisesame IgG, IgA and IgE-responses were observed in the serum of sensitized individuals, different from the lower signals obtained with control samples. Western blot analysis demonstrated highl...Continue Reading

Citations

Jul 5, 2003·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·N WolffS Yannai
Apr 7, 2006·Annals of Allergy, Asthma & Immunology : Official Publication of the American College of Allergy, Asthma, & Immunology·UNKNOWN American College of Allergy, Asthma, & Immunology
Aug 13, 2005·Annals of Allergy, Asthma & Immunology : Official Publication of the American College of Allergy, Asthma, & Immunology·Venu GangurLalitha Navuluri
Aug 23, 2002·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·S FremontJ P Nicolas
Nov 6, 2004·International Archives of Allergy and Immunology·Isabella Schöll, Erika Jensen-Jarolim
May 16, 2006·International Archives of Allergy and Immunology·Lalitha NavuluriVenu Gangur
Oct 10, 2006·Biotechnology Letters·Estibalitz Orruño, Michael R A Morgan

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