PMID: 11340876May 9, 2001Paper

Specific hydraulic conductivity of corneal stroma as seen by quick-freeze/deep-etch

Journal of Biomechanical Engineering
Darryl R OverbyM Johnson

Abstract

Previous studies of the hydraulic conductivity of connective tissues have failed to show a correspondence between ultrastructure and specific hydraulic conductivity. We used the technique of quick-freeze/deep-etch to examine the ultrastructure of the corneal stroma and then utilized morphometric studies to compute the specific hydraulic conductivity of the corneal stroma. Our studies demonstrated ultrastructural elements of the extracellular matrix of the corneal stroma that are not seen using conventional electron microscopic techniques. Furthermore, we found that these structures may be responsible for generating the high flow resistance characteristic of connective tissues. From analysis of micrographs corrected for depth-of-field effects, we used Carmen-Kozeny theory to bound a morphometrically determined specific hydraulic conductivity of the corneal stroma between 0.46 x 10(-14) and 10.3 x 10(-14) cm2. These bounds encompass experimentally measured values in the literature of 0.5 x 10(-14) to 2 x 10(-14) cm2. The largest source of uncertainty was due to the depth-of-field estimates that ranged from 15 to 51 nm; a better estimate would substantially reduce the uncertainty of these morphometrically determined values.

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Citations

Jul 21, 2012·Pharmaceutical Research·Chhavi GuptaSangly P Srinivas
May 1, 2010·Langmuir : the ACS Journal of Surfaces and Colloids·Ramin ZareianJeffrey W Ruberti
Apr 27, 2007·Annual Review of Biomedical Engineering·Melody A Swartz, Mark E Fleury
Jun 7, 2008·Journal of Applied Physiology·William J McCartyMark Johnson
Feb 26, 2008·Investigative Ophthalmology & Visual Science·Jiahn-Dar HuangMark Johnson
Jul 22, 2015·Computer Methods in Biomechanics and Biomedical Engineering·Avinash AyyalasomayajulaJonathan P Vande Geest
Jun 26, 2007·Experimental Eye Research·Jiahn-Dar HuangMark Johnson
Dec 21, 2005·Journal of Microscopy·Darryl R Overby, Mark Johnson
Jan 3, 2006·Experimental Eye Research·Mark Johnson
Jul 31, 2013·Experimental Eye Research·Hamed Hatami-Marbini, Ebitimi Etebu
Jul 17, 2004·Annual Review of Biomedical Engineering·C Ross EthierJeff Ruberti
Nov 4, 2009·Acta Biomaterialia·Hyungsuk LeeRoger D Kamm
Dec 6, 2018·Biomaterials Science·S K RanamukhaarachchiS I Fraley
Aug 30, 2008·American Journal of Physiology. Cell Physiology·Charles W McLaughlinMortimer M Civan
Apr 4, 2008·American Journal of Physiology. Cell Physiology·William M DismukeDorette Z Ellis

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