Specific Identification of Glycoproteins Bearing the Tn Antigen in Human Cells

Angewandte Chemie
Jiangnan ZhengRonghu Wu

Abstract

Glycoproteins contain a wealth of valuable information regarding the development and disease status of cells. In cancer cells, some glycans (such as the Tn antigen) are highly up-regulated, but this remains largely unknown for glycoproteins with a particular glycan. Herein, an innovative method combining enzymatic and chemical reactions was first designed to enrich glycoproteins with the Tn antigen. Using synthetic glycopeptides with O-GalNAc (the Tn antigen) or O-GlcNAc, we demonstrated that the method is selective for glycopeptides with O-GalNAc and can distinguish between these two modifications. The diagnostic ions from the tagged O-GalNAc further confirmed the effectiveness of the method and confidence in the identification of glycopeptides with the Tn antigen by mass spectrometry. Using this method, we identified 96 glycoproteins with the Tn antigen in Jurkat cells. The method can be extensively applied in biological and biomedical research.

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Citations

Oct 11, 2017·Journal of Separation Science·Xin YouMingliang Ye
Apr 19, 2018·Journal for Immunotherapy of Cancer·Laura L EgginkJ Kenneth Hoober
Feb 29, 2020·Chemical Science·Lijun YangHaojie Lu
Jul 12, 2020·International Journal of Molecular Sciences·J Kenneth Hoober
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Feb 16, 2021·Molecular & Cellular Proteomics : MCP·Nicholas M RileySharon J Pitteri
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May 29, 2021·Mass Spectrometry Reviews·Fangxu SunRonghu Wu
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Oct 19, 2019·Analytical Chemistry·Suttipong SuttapitugsakulRonghu Wu

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