Specific immunoglobulin M enzyme-linked immunosorbent assay for confirming the diagnosis of measles.

Journal of Clinical Microbiology
A W Lievens, P A Brunell

Abstract

An enzyme-linked immunosorbent assay (ELISA) was evaluated for the detection of measles virus-specific immunoglobulin M (IgM) (MIgM). The ELISA was standardized by deriving a seronegative range of values from sera which should not contain MIgM (24 cord sera, 59 sera from immune health care workers, and 47 sera from infants before the administration of measles vaccine). These values were separable from those obtained from individuals convalescing from measles. Twenty sera containing rheumatoid factor were MIgM seronegative. Of 30 acute-phase sera from suspected measles cases, 26 contained MIgM; those that were seronegative were obtained on day 0, 0, 2, or 9. All 25 convalescent-phase samples contained MIgM. Of the 25 paired samples, 22 were IgG positive at the first sampling; 3 of the 22 did not show a rise in IgG titer. The MIgM ELISA can be used for confirming suspected measles cases, often requiring only a single serum specimen.

References

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Citations

Jul 20, 2007·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Philip Brunell
Mar 26, 1987·The New England Journal of Medicine·T L GustafsonL M Sehulster
Nov 15, 1990·Postgraduate Medicine·K M Ramsey
Sep 1, 1995·Revista Do Instituto De Medicina Tropical De São Paulo·S A OliveiraJ P Nascimento
May 2, 2003·The Journal of Infectious Diseases·Linda VenczelCiro de Quadros
Dec 1, 1991·Journal of Clinical Microbiology·D R MayoP Lamb

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