Specific immunohistochemical detection of the BRAF V600E mutation in primary and metastatic papillary thyroid carcinoma

Experimental and Molecular Pathology
Xiaoli ZhuXiaoyan Zhou

Abstract

To evaluate the clinical value of immunohistochemistry (IHC) using anti-BRAF V600E antibody (clone VE1) for specific detection of the BRAF V600E mutant protein in formalin-fixed paraffin-embedded papillary thyroid carcinoma (PTC) specimens. A total of 118 PTC cases and 116 control cases processed between January 2008 and June 2010 were selected and created tissue microarrays (TMAs) for the study. BRAF V600E (VE1) IHC was performed on tissue sections from PTC cases to determine mutation status. Molecular tests (Sanger sequencing/ARMS) were used to confirm the BRAF V600E gene mutation in primary PTC. A uniformly cytoplasmic staining throughout the tumors was observed in IHC-positive cases. BRAF V600E was detected in 68.6% (81/118) of PTC samples by IHC and in 61.9% (73/118) by Sanger sequencing/ARMS. The overall concordance between IHC and Sanger sequencing/ARMS was 93.2% (110/118). The sensitivity and specificity of the BRAF V600E IHC was 100% (73/73) and 82.2% (37/45), respectively. Positive and negative predictive values were 90.1% (73/81) and 100% (37/37), respectively. Expression of the BRAF V600E mutant protein was detected in all of 59 cases of primary carcinoma and corresponding metastatic carcinoma in lymph nodes. The co...Continue Reading

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Citations

Jul 25, 2019·Current Hematologic Malignancy Reports·Narittee Sukswai, Joseph D Khoury
Dec 12, 2020·Journal of Clinical Laboratory Analysis·Faiza A RashidSyed Sameer Aga
Dec 10, 2020·Fetal and Pediatric Pathology·Dang Anh Thu PhanThanh Tung Tran
Nov 7, 2020·Indian Journal of Pathology & Microbiology·Sang Ah LeeYoung Hee Maeng

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