Specific immunoneutralization of FSH leads to apoptotic cell death of the pachytene spermatocytes and spermatogonial cells in the rat

Endocrinology
J ShettyR R Dighe

Abstract

Although requirement for follicle stimulating hormone (FSH) in the initiation of spermatogenesis is well documented, its role in adult spermatogenesis is still debated. In the present communication, we have investigated the effect of specific immunoneutralization of FSH on apoptotic cell death in the testicular germ cells both in immature and adult rats. The germ cells of control animals showed predominantly high molecular weight DNA while the antiserum (a/s) treated group showed DNA fragmentation characteristic of apoptosis. The pattern could be detected within 24 hours of a/s treatment, and became more pronounced after 48 hours. The germ cells were purified from FSH a/s treated rats by centrifugal elutriation and vulnerability of each cell type to undergo apoptosis on FSH neutralization was investigated. The pachytene spermatocytes were found to be most sensitive to absence of FSH, even in the adult animals suggesting the involvement of FSH in spermatogenesis. The in situ analysis of DNA strand breakage following FSH a/s treatment showed fragmentation of the DNA of the pachytene spermatocytes confirming this observation. The in situ analysis also showed that the spermatogonia undergo apoptosis in addition to the pachytene spe...Continue Reading

Citations

Apr 25, 2009·American Journal of Physiology. Gastrointestinal and Liver Physiology·Romina MancinelliGianfranco Alpini
Jul 9, 2004·Cytogenetic and Genome Research·S S W Chung, D J Wolgemuth
Apr 26, 2008·Endocrine Reviews·Stephanie T PageWilliam J Bremner
Oct 25, 2013·Molecular and Cellular Endocrinology·Cristina CoccoBarbara Noli
May 4, 2011·International Journal of Andrology·R Walczak-JedrzejowskaJ Slowikowska-Hilczer
Aug 15, 2006·The Journal of Steroid Biochemistry and Molecular Biology·Ayesha R Joshi, Rajan R Dighe
May 20, 2005·Differentiation; Research in Biological Diversity·Sanny S W ChungDebra J Wolgemuth
Mar 18, 2009·Microscopy Research and Technique·Marie-Hélène Perrard, Philippe Durand
Feb 3, 2007·Apoptosis : an International Journal on Programmed Cell Death·Tej K PareekRajan R Dighe
Dec 10, 2009·Folia Histochemica Et Cytobiologica·Hashemitabar MahmoudAlbughobeish Naeim
Jul 16, 2010·Andrologia·M OrazizadehM Hashemitabar
Nov 1, 2001·Journal of Endocrinological Investigation·M K Gill-SharmaH S Juneja
Aug 10, 2006·The Journal of Clinical Endocrinology and Metabolism·Kati L MatthiessonSarah J Meachem
Nov 7, 1998·International Journal of Andrology·J Blanco-Rodríguez
Oct 14, 2005·Toxicological Sciences : an Official Journal of the Society of Toxicology·Michael G WadeIh Chu
Aug 27, 2010·Biology of Reproduction·Jitu W GeorgeLeslie L Heckert
Jul 7, 2015·Reproduction, Fertility, and Development·Carlos Vladimir Herrera-LunaChristine Aurich
Jan 9, 2019·Frontiers in Endocrinology·Monica Muratori, Elisabetta Baldi
Mar 6, 2019·Frontiers in Endocrinology·Elodie KaraMarie-Christine Maurel
Nov 17, 2019·Animals : an Open Access Journal From MDPI·Zhicheng ShiFangxiong Shi
Nov 3, 2012·Pharmaceutical Biology·Layasadat KhorsandiSoheila Khaghani
Apr 30, 2021·Saudi Journal of Biological Sciences·Sara Mohamed SaberAmel Ramadan Omar
May 1, 2021·Biology of Reproduction·Millissia Ben MaamarMichael K Skinner

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.