Specific impairment of endothelium-derived hyperpolarizing factor-type relaxation in mesenteric arteries from streptozotocin-induced diabetic mice

Vascular Pharmacology
Takayuki MatsumotoKatsuo Kamata

Abstract

We hypothesized that the contribution made by endothelium-derived hyperpolarizing factor (EDHF) to acetylcholine (ACh)-induced endothelium-dependent relaxation (EDR) might be altered in mesenteric arteries from streptozotocin (STZ)-induced diabetic mice. In endothelium-intact preparations, the ACh-induced EDR (but not the sodium nitroprusside-induced relaxation) was weaker in the STZ group than in age-matched controls. Indomethacin (10 muM) had no significant effect on EDR in either group, indicating that cyclooxygenase products, including prostacyclin, are not involved. This indomethacin-resistant EDR was weaker in the STZ group than in the controls. To isolate the EDHF-resistant component of EDR, charybdotoxin (100 nM) and apamin (100 nM) were present in the bath solution throughout the next experiment. This EDHF-resistant relaxation did not differ significantly between the two groups. On the other hand, the EDHF-mediated relaxation was significantly weaker in the STZ group than in the controls, and it was completely blocked by lysophosphatidylcholine (LPC, 10 microM) in each group. The eNOS protein expression was similar between the two groups. These results suggest that (a) the endothelial dysfunction present in mesenteric ...Continue Reading

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Citations

Dec 13, 2006·Journal of Smooth Muscle Research = Nihon Heikatsukin Gakkai Kikanshi·Katsuo KamataTsuneo Kobayashi
May 9, 2008·American Journal of Physiology. Cell Physiology·Ayako MakinoWolfgang H Dillmann
Sep 10, 2008·Vascular Pharmacology·Joanna L StanleyPhilip N Baker
Jul 29, 2008·Atherosclerosis·Leanid LukshaKarolina Kublickiene
Sep 27, 2006·Journal of Smooth Muscle Research = Nihon Heikatsukin Gakkai Kikanshi·Katsuo KamataToshio Honda
May 25, 2007·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Sharyn M FitzgeraldRoger G Evans
Jun 21, 2015·American Journal of Physiology. Heart and Circulatory Physiology·Natalia I GokinaGabriela Goloman
Aug 17, 2014·American Journal of Physiology. Heart and Circulatory Physiology·Christian SchachDierk H Endemann
Aug 3, 2019·International Journal of Molecular Sciences·Kenichi Goto, Takanari Kitazono
Oct 7, 2010·Journal of Diabetes·Yi Shi, Paul Michel Vanhoutte

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