Abstract
Prothymosin alpha (ProTα) is a conserved mammalian polypeptide with intracellular functions associated with cell proliferation and apoptosis and an extracellular role associated with immunopotentiation. The N-terminal fragment [1-28], which is identical with the immunostimulating peptide thymosin α1 (Tα1), was earlier considered as the immunoactive region of the polypeptide; however, recent data suggest that ProTα may exert a discrete immunomodulating action through its central or C-terminal region, via targeting Toll-like receptor- 4 (TLR4). In this work, a derivative of the C-terminal fragment ProTα[100-109] (ProTα-D1) that can be radiolabeled with (99m)Tc was developed. The biological activity of the non-radioactive (185/187)rhenium-complex of this derivative ([(185/187)Re]ProTα-D1, structurally similar with [(99m)Tc]ProTα-D1) was verified through suitable in vitro bioassays on human neutrophils. Subsequent cell-binding studies revealed specific, time-dependent and saturable binding of [(99m)Tc]ProTα-D1 on neutrophils, which was inhibited by intact ProTα and ProTα[100-109], as well as by a "prototype" TLR4-ligand (lipopolysaccharide from Escherichia coli). Overall, our results support the existence of ProTα-binding sites on ...Continue Reading
References
Jan 1, 1979·Annals of the New York Academy of Sciences·T L LowA L Goldstein
Dec 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·W H Eschenfeldt, S L Berger
Jan 1, 1986·International Journal of Radiation Applications and Instrumentation. Part B, Nuclear Medicine and Biology·E DeutschH R Maxon
Sep 1, 1966·Proceedings of the National Academy of Sciences of the United States of America·A L GoldsteinA White
Feb 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·A A HaritosB L Horecker
Aug 1, 1995·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·O J CorderoM Nogueira
Mar 17, 2000·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·M D CarvalhoE C Quintão
Nov 10, 2000·Peptides·A PiñeiroM Nogueira
Dec 1, 2001·Expert Opinion on Biological Therapy·B Bodey
Dec 4, 2001·Biochimica Et Biophysica Acta·V KoutrafouriG P Evangelatos
Mar 22, 2002·British Journal of Pharmacology·Sigrid WittmannStephen Daniels
Jan 11, 2003·Science·Xuejun JiangXiaodong Wang
Mar 26, 2003·Experimental Cell Research·Alexandra G EvstafievaAndrey B Vartapetian
Jun 28, 2003·Blood·Fumitaka HayashiAndrew D Luster
Dec 31, 2003·Nuclear Medicine and Biology·C J SmithT J Hoffman
Feb 26, 2004·Blood·Luigina RomaniEnrico Garaci
Jul 2, 2004·Nature Reviews. Immunology·Shizuo Akira, Kiyoshi Takeda
Feb 3, 2006·Cancer Immunology, Immunotherapy : CII·Margarita SkopelitiOurania E Tsitsilonis
Sep 2, 2006·Molecular Biotechnology·Muriel AmblardGilles Subra
Nov 18, 2006·Journal of Viral Hepatitis·R-N ChienY-F Liaw
Jul 17, 2007·Chemical Biology & Drug Design·Persefoni KlimentzouChristos Zikos
Jul 4, 2009·Chemistry : a European Journal·Ramon Subirós-FunosasFernando Albericio
Aug 3, 2011·Future Medicinal Chemistry·Arevik Mosoian
Mar 1, 2012·Cancer Immunology, Immunotherapy : CII·Kyriaki IoannouOurania E Tsitsilonis
Jun 14, 2012·Chemical Biology & Drug Design·Dimitra BenakiEvangelia Livaniou
Jan 2, 2013·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Ioannis F VoutsasAristotelis Bamias
Jan 5, 2013·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Kazutoshi SaitoNaohiro Nishiyama
Citations
Feb 16, 2017·Journal of Cellular Biochemistry·Yi FanLingmei Qian
Jan 19, 2016·Neuroscience·Hiroshi UedaS Maeda
Jan 15, 2017·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Chrysoula-Evangelia KarachaliouEvangelia Livaniou
Jun 17, 2016·PloS One·G Luca GusellaArevik Mosoian
Nov 14, 2019·Heliyon·Chrysoula-Evangelia KarachaliouEvangelia Livaniou