Specific incorporation of heat shock protein 70 family members into primate lentiviral virions

Journal of Virology
Cagan GurerJeremy Luban

Abstract

To determine if any heat shock proteins are incorporated into human immunodeficiency virus type 1 (HIV-1) virions in a manner similar to that of the peptidyl-prolyl isomerase cyclophilin A, we probed purified virions with antibodies against heat shock proteins Hsp27, Hsp40, Hsp60, Hsp70, Hsc70, and Hsp90. Of these proteins, Hsp60, Hsp70, and Hsc70 associated with virions purified based on either particle density or size and were shown to be incorporated within the virion membrane, where they were protected from digestion by exogenous protease. Virion incorporation of Hsp70 was also observed with HIV-2 and with simian immunodeficiency viruses SIV(MAC) and SIV(AGM), but it appears to be specific for primate lentiviruses, since Hsp70 was not detected in association with Moloney murine leukemia virus virions. Of the HIV-1 genes, gag was found to be sufficient for Hsp70 incorporation, though Hsp70 was roughly equimolar with pol-encoded proteins in virions.

References

Jan 2, 1992·Nature·M J Gething, J Sambrook
Dec 1, 1995·Journal of Virology·T P CripeR L Garcea
Jun 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·K SykesJ Sambrook
Feb 1, 1994·Journal of Medical Primatology·S R BartzM Malkovsky
Feb 6, 1996·Proceedings of the National Academy of Sciences of the United States of America·J Hu, C Seeger
Apr 3, 1998·Journal of Virology·R A WeldonE Hunter
Nov 13, 1999·Journal of Virology·Y TangH C Morse
Oct 29, 2000·AIDS Research and Human Retroviruses·W RaddingJ M McDonald
Jun 28, 2001·Proceedings of the National Academy of Sciences of the United States of America·L VerPlankC A Carter

❮ Previous
Next ❯

Citations

Nov 1, 2002·Reviews in Medical Virology·David E Ott
Jun 23, 2009·Journal of Clinical Immunology·Jessica L ReynoldsStanley A Schwartz
Jan 4, 2013·Journal of Proteome Research·Cameron J SchweitzerMichael Belshan
Feb 28, 2007·Nature Reviews. Microbiology·Stephen P Goff
Apr 30, 2004·The New England Journal of Medicine·Justin StebbingDaniel C Douek
Jan 8, 2010·The Journal of Biological Chemistry·Chae Young HwangByeongwoon Song
Sep 3, 2010·The Journal of Biological Chemistry·Seiga OhmineYasuhiro Ikeda
Jan 14, 2011·The Journal of Biological Chemistry·Ryuichi SugiyamaHiroshi Takaku
May 14, 2004·Journal of Virology·Luise FlorinMartin Sapp
May 14, 2005·Journal of Virology·Réjean CantinMichel J Tremblay
Dec 23, 2004·Journal of Virology·Cagan GurerJeremy Luban
Mar 30, 2005·Journal of Virology·Jill T BechtelDon Ganem
Apr 14, 2005·Journal of Virology·Cagan GurerJeremy Luban
Mar 31, 2006·Journal of Virology·Elena FrolovaIlya Frolov
Jun 11, 2010·Proteome Science·Qingming KongYongchang Cao
Mar 21, 2006·Retrovirology·Alan W CochraneAndrew J Mouland
Jul 27, 2007·Retrovirology·Juan Lama, Vicente Planelles
Sep 6, 2011·Retrovirology·Charline GiroudLaurence Briant
Aug 15, 2012·Retrovirology·Steven SantosSergey Iordanskiy
Oct 14, 2009·Virology Journal·Megan Moerdyk-SchauweckerValery Z Grdzelishvili
Jun 26, 2008·PloS One·Tong WangHoward E Gendelman
Jul 19, 2012·PloS One·Cameron J SchweitzerMichael Belshan
Jun 7, 2008·PLoS Pathogens·Megan L ShawPeter Palese
Jul 9, 2011·PLoS Pathogens·Fengwen ZhangPaul D Bieniasz
Jul 19, 2012·PLoS Pathogens·Ferdinand RoeschOlivier Schwartz
Jan 1, 2007·Future Virology·Sergey Iordanskiy, Michael Bukrinsky
Oct 4, 2012·Future Microbiology·Dimitrios ChatzidimitriouNikolaos Malisiovas
May 7, 2004·Proceedings of the National Academy of Sciences of the United States of America·Shakti Narayan, John A T Young
Aug 30, 2003·Proceedings of the National Academy of Sciences of the United States of America·Stephen J GouldJames E K Hildreth

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.