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Specific inhibition of Raf-1 gene expression in HNE1 by RNA interference

Zhonghua er bi yan hou tou jing wai ke za zhi = Chinese journal of otorhinolaryngology head and neck surgery

Feb 8, 2007

Li-Zhong LiuBing Kan

PMID: 17283530

Abstract

To silence the expression of Raf-1 gene in HNE1 cells using vector-based RNA interference (RNAi) technique. The vector containing the human U6 promoter was used for targeted gene silencing when a dsDNA oligonucleotide encoding an appropriate shRNA was ligated into the vector, and 67nt o...read more

Mentioned in this Paper

Cancer of Nasopharynx
Shuttle Vectors
Transfection
Apoptosis, Intrinsic Pathway
Cosuppression
Proto-Oncogene Proteins c-raf
Antisense RNA
Promoter
Reverse Transcriptase Polymerase Chain Reaction
Gene Expression
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Specific inhibition of Raf-1 gene expression in HNE1 by RNA interference

Zhonghua er bi yan hou tou jing wai ke za zhi = Chinese journal of otorhinolaryngology head and neck surgery

Feb 8, 2007

Li-Zhong LiuBing Kan

PMID: 17283530

DOI:

Abstract

To silence the expression of Raf-1 gene in HNE1 cells using vector-based RNA interference (RNAi) technique. The vector containing the human U6 promoter was used for targeted gene silencing when a dsDNA oligonucleotide encoding an appropriate shRNA was ligated into the vector, and 67nt o...read more

Mentioned in this Paper

Cancer of Nasopharynx
Shuttle Vectors
Transfection
Apoptosis, Intrinsic Pathway
Cosuppression
Proto-Oncogene Proteins c-raf
Antisense RNA
Promoter
Reverse Transcriptase Polymerase Chain Reaction
Gene Expression

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RNA Interference (RNAi) pathway is involved in post-transcriptional gene silencing, transcriptional silencing and epigenetic silencing as well as its use as a tool for forward genetics and therapeutics. Discover the latest research here.

RNA Hairpin in Yeast

Short hairpin RNA are used to interrogate gene function in model organisms. Discover the latest research in RNA hairpins in Yeast.

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Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition

Inhibiting E7 oncogene in CaSki cells of cervical cancer by vector-based RNA interference technique

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Paper Details
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