PMID: 3320654Dec 28, 1987Paper

Specific insulin binding in bovine chromaffin cells; demonstration of preferential binding to adrenalin-storing cells

Life Sciences
G Serck-Hanssen, O Søvik

Abstract

Insulin binding was studied in subpopulations of bovine chromaffin cells enriched in adrenalin-producing cells (A-cells) or noradrenalin-producing cells (NA-cells). Binding of 125I-insulin was carried out at 15 degrees C for 3 hrs in the absence or presence of excess unlabelled hormone. Four fractions of cells were obtained by centrifugation on a stepwise bovine serum albumin gradient. The four fractions were all shown to bind insulin in a specific manner and the highest binding was measured in the cell layers of higher densities, containing mainly A-cells. The difference in binding of insulin to the four subpopulations of chromaffin cells seemed to be related to differences in numbers of receptors as opposed to receptor affinities. We conclude that bovine chromaffin cells possess high affinity binding sites for insulin and that these binding sites are mainly confined to A-cells.

References

Aug 1, 1986·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·M O'Connor-McCourtM D Hollenberg
Mar 14, 1986·Biochimica Et Biophysica Acta·L Bigornia, I Bihler
Nov 1, 1982·Cell·S M BlockH C Berg
Apr 17, 1980·The New England Journal of Medicine·O PedersenL Heding
May 1, 1981·Experimental Cell Research·S P Wilson, O H Viveros
Oct 1, 1963·Proceedings of the Society for Experimental Biology and Medicine·R C CANTUW F GANONG

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Citations

Jan 1, 1988·The International Journal of Biochemistry·G Serck-HanssenR T Lie

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