Specific interaction between Sam68 and neuronal mRNAs: implication for the activity-dependent biosynthesis of elongation factor eEF1A.

Journal of Neuroscience Research
Julien GrangeYves Goldberg

Abstract

In cultured hippocampal neurons and in adult brain, the splicing regulatory protein Sam68 is partially relocated to the somatodendritic domain and associates with dendritic polysomes. Transfer to the dendrites is activity-dependent. We have investigated the repertoire of neuronal mRNAs to which Sam68 binds in vivo. By using coimmunoprecipitation and microarray screening techniques, Sam68 was found to associate with a number of plasticity-related mRNA species, including Eef1a1, an activity-responsive mRNA coding for translation elongation factor eEF1A. In cortical neuronal cultures, translation of the Eef1a1 mRNA was strongly induced by neuronal depolarisation and correlated with enhanced association of Sam68 with polysomal mRNAs. The possible function of Sam68 in Eef1a1 mRNA utilization was studied by expressing a dominant-negative, cytoplasmic Sam68 mutant (GFP-Sam68DeltaC) in cultured hippocampal neurons. The level of eEF1A was lower in neurons expressing GFP-Sam68DeltaC than in control neurons, supporting the proposal that endogenous Sam68 may contribute to the translational efficiency of the Eef1a1 mRNA. These findings are discussed in the light of the complex, potentially crucial regulation of eEF1A biosynthesis during lon...Continue Reading

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Citations

Feb 6, 2013·Proceedings of the National Academy of Sciences of the United States of America·Matthew E KleinBryen A Jordan
Jul 9, 2010·Cold Spring Harbor Perspectives in Biology·Maria Carmo-FonsecaMiguel Lafarga
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Jul 20, 2010·RNA Biology·Roberta Busà, Claudio Sette
Jul 21, 2009·Experimental Cell Research·Jorge Henao-Mejia, Johnny J He

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