PMID: 9168157Apr 26, 1997Paper

Specific interaction between tetrandrine and Quillaja saponins in promoting permeabilization of plasma membrane in human leukemic HL-60 cells

Biochimica Et Biophysica Acta
Y M LeungT T Loh

Abstract

Spontaneous Ni2+ entry (leak), measured as fluorescence quench in fura-2-loaded HL-60 cells at the excitation wavelength of 360 nm, was strongly inhibited by tetrandrine (TET, 100 microM), a Ca2+ antagonist of Chinese herbal origin. Exposure of the cells for 5 min to saponins from Quillaja saponaria (QS, 30 microg/ml), surfactants well known to permeabilize the plasma membrane by complexing with cholesterol, promoted Ni2+ entry without causing fura-2 leak-out. Unexpectedly, TET caused an immediate (within 2.5 min) augmentation of QS-promoted Ni2+ entry; and a 5-min treatment with both TET and QS resulted not only in an enhanced Ni2+ entry, but also a fura-2 leak-out. Ginseng saponins (100 microg/ml) alone or together with TET did not cause such a permeabilization. Permeabilization induced by 1-3 microM digitonin, another cholesterol-complexing glycoside, could not be enhanced by TET. TET did not affect permeabilization induced by Triton X-100 (0.01%), a detergent which non-specifically disrupts the hydrophobic interaction at the plasma membrane. TET also did not enhance Ni2+ entry triggered by ionomycin (0.35 microM) or SK&F 96365 (20 microM). Further, it did not augment Ni2+ entry when the plasma membrane fluidity was modulate...Continue Reading

References

Jan 1, 1990·International Journal of Immunopharmacology·B S TehY H Thong
Nov 15, 1995·The Biochemical Journal·M J Berridge

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Citations

Apr 14, 2012·Journal of Biomedicine & Biotechnology·Michał ArabskiWiesław Kaca
Jun 25, 2002·Toxicology and Applied Pharmacology·Sung Jin HwangJong Kun Park
Dec 9, 2020·Animals : an Open Access Journal From MDPI·Hernán Cañon-JonesRicardo San Martín

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