Specific interaction of food proteins with apical membranes of the human intestinal cell lines Caco-2 and T84

Clinica Chimica Acta; International Journal of Clinical Chemistry
G BolteM Stern

Abstract

A comparison between the intestinal epithelial cell lines Caco-2 and T84 was made to assess the influence of enterocytic differentiation on food protein binding capacities of the brush border membrane. Cell morphology and expression of brush border-associated enzymes were studied as differentiation markers. Food protein binding to isolated brush border membranes was measured with a dot blot chemiluminescence assay. Early at confluence, Caco-2 cells exhibited a more differentiated state compared to T84 cells. Brush border membranes of both cell lines bound gliadin peptides, beta-lactoglobulin and ovalbumin specifically. Binding capacities increased from gliadin peptides to ovalbumin to beta-lactoglobulin. There was correlation of membrane binding capacity with degree of cell differentiation. Due to their similarity to small intestinal epithelial cells, the colon carcinoma cell lines Caco-2 and T84 represent models for studying food protein-enterocytic brush border membrane interactions in relation to varying degrees of cell differentiation.

References

Dec 12, 1991·The New England Journal of Medicine·J S Trier
May 1, 1990·Journal of Cellular Physiology·M HeymanJ F Desjeux
Aug 1, 1989·European Journal of Immunology·D KaiserlianJ P Revillard
Dec 1, 1985·The Journal of Cell Biology·J L Madara, K Dharmsathaphorn
Sep 27, 1973·Biochimica Et Biophysica Acta·J SchmitzR K Crane
Jul 1, 1969·Analytical Biochemistry·H Eibl, W E Lands
Jan 15, 1994·European Journal of Biochemistry·H P HauriH Naim
Nov 1, 1994·Pediatric Research·K PittschielerJ K Petell
Jun 1, 1996·The Journal of Membrane Biology·D T ThwaitesN L Simmons
Mar 29, 1996·Clinica Chimica Acta; International Journal of Clinical Chemistry·G BolteM Stern
Dec 1, 1996·Gastroenterology·H C ReineckerD K Podolsky

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Citations

Aug 13, 1999·Journal of Periodontal Research·J MeyleH Wolburg
Nov 10, 2009·Bioscience, Biotechnology, and Biochemistry·Tetsunosuke MochizukiMakoto Shimizu
Aug 29, 2013·In Vitro Cellular & Developmental Biology. Animal·Pauline BeguinYvan Larondelle
Dec 1, 2004·Journal of Pharmaceutical Sciences·Phani Prasanth Tirumalasetty, John G Eley
Nov 15, 2014·Journal of Tissue Engineering·Debra FranckJoshua R Mauney
Mar 8, 2017·Histochemistry and Cell Biology·Sarah DevrieseDebby Laukens
Jun 21, 2019·International Journal of Molecular Sciences·Sylvester LarsenJesper T Troelsen

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