PMID: 6965942Apr 1, 1980Paper

Specific interaction of murine colony-stimulating factor with mononuclear phagocytic cells

The Journal of Cell Biology
L J Guilbert, E R Stanley

Abstract

L-cell colony-stimulating factor (CSF) is identical to macrophage growth factor and stimulates macrophage proliferation (Stanley et al., 1976, J. Exp. Med. 143: 631-647). The nature of the interaction of iodinated L-cell CSF (125I-CSF) with murine peritoneal exudate macrophages was studied. On incubation with 10 pM 125I-CSF at 0 degrees C, cellular binding of 125I-CSF reaches a stable maximum within 15 h. This is in contrast to the association behavior at higher temperatures. At 37 degrees C, cell-associated 125I-CSF levels reach, within 45 min, an unstable maximum which is up to 10-fold less than that occurring under the same conditions at 0 degrees C. At 0 degrees C, binding is saturated (approximately 5 X 10(4) sites/cell) at CSF concentrations of 1 nM. A comparison of binding and competition experiments indicates that iodinated L-cell CSF binds as effectively as L-cell CSF and that human urinary CSF and L-cell CSF equipotently compete for 125I-CSF binding. Specificity of the CSF-binding site is demonstrated by the failure of other known growth factors and hormones to compete for 125I-CSF binding. These studies and other findings suggest that 125I-CSF binding is restricted to macrophages and their precursors and to macrophag...Continue Reading

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Citations

Oct 1, 1993·Journal of Cellular Biochemistry·T MeenakshiS L Teitelbaum
Jul 1, 1994·Journal of Cellular Biochemistry·R FelixH Fleisch
Apr 1, 1992·Journal of Cellular Physiology·A UmezawaJ Hata
Nov 1, 1990·Journal of Neuroscience Research·C HaoS Fedoroff
Feb 1, 1994·Molecular and Chemical Neuropathology·R L Alterman, E R Stanley
May 1, 1989·Developmental Biology·L J Regenstreif, J Rossant
Oct 12, 1984·Journal of Immunological Methods·L J Guilbert, E R Stanley
Apr 6, 2004·Developmental and Comparative Immunology·Daniel R BarredaMiodrag Belosevic
Jun 12, 2013·Developmental and Comparative Immunology·Barbara A KatzenbackMiodrag Belosevic
Jun 7, 2005·Fish & Shellfish Immunology·Miodrag BelosevicDaniel R Barreda
Jun 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·N A Nicola, D Metcalf
May 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·R SaccaC W Rettenmier
Feb 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·J C GassonD W Golde
Sep 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·A BartocciE R Stanley
Mar 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·Y G YeungE R Stanley
Aug 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·D J HiltonD Metcalf
Jun 1, 1987·The Journal of Clinical Investigation·C A Sieff
Mar 1, 1989·The Journal of Clinical Investigation·S RamakrishnanE L Brown
Jan 7, 2006·Blood·Johannes GrosseE Richard Stanley
Jun 24, 2010·Journal of Leukocyte Biology·Saskia GuellerH Phillip Koeffler
Jan 13, 2006·Proceedings of the National Academy of Sciences of the United States of America·Robert B WestMatt van de Rijn
Dec 4, 2015·Biology·Jordan W HodgkinsonMiodrag Belosevic

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