Specific involvement of glypican in thrombin adhesive properties

Journal of Cellular Biochemistry
R Bar-ShavitI Vlodavsky

Abstract

We have previously demonstrated that thrombin possesses an active yet cryptic Arg-Gly-Asp (RGD) site which upon exposure induces endothelial cell (EC) adhesion via alpha nu beta 3 integrin [Bar-Shavit et al. (1991): J Cell Biol 112:335]. This was achieved in the presence of cell surface-associated heparan sulfate proteoglycans (HSPG) and exceedingly low concentrations of plasmin [Bar-Shavit et al. (1993): J Cell Biol 123:1279]. A portion of the cell surface-associated HSPG (glypican) is anchored via a covalently linked glycosyl-phosphatidylinositol (PI) residue, which can be released by treatment with glycosyl-PI-specific phospholipase C (PI-PLC). We report here that exposure of either bovine aortic EC, smooth muscle cells (SMC), or wild-type CHO cells to PI-PLC released HSPG involved in the conversion of thrombin to an adhesive molecule. The adhesion-promoting activity of the released HSPG was abolished following treatment with heparinase but not chondroitinase ABC. Incubation of thrombin with heparan sulfate-deficient CHO cells or cells that were pretreated with PI-PLC failed to induce its conversion to an adhesive molecule, indicating that glypican was playing a major role in this conversion. Moreover, affinity-purified glyp...Continue Reading

References

Nov 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·D GospodarowiczC Birdwell
Oct 1, 1992·Protein Science : a Publication of the Protein Society
Feb 1, 1992·The Journal of Clinical Investigation·S R CoughlinV I Wheaton
Feb 1, 1992·American Journal of Respiratory Cell and Molecular Biology·R Bar-ShavitI Vlodavsky
Jan 23, 1992·The New England Journal of Medicine·V FusterJ H Chesebro
Oct 1, 1991·Current Opinion in Cell Biology·J D Esko
Jan 1, 1991·The Journal of Cell Biology·R Bar-ShavitE Dejana
Jul 1, 1990·The Journal of Membrane Biology·M P LisantiA R Saltiel
Jan 1, 1989·Arteriosclerosis : an Official Journal of the American Heart Association, Inc·A D Cardin, H J Weintraub
Oct 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·D J Fitzgerald, G A Fitzgerald
Apr 20, 1989·The New England Journal of Medicine·R H Eckel
Oct 1, 1989·The Journal of Clinical Investigation·R Bar-ShavitI Vlodavsky
Jan 1, 1986·Annals of the New York Academy of Sciences·M A Shuman
May 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·J D EskoW H Taylor
Mar 1, 1984·Analytical Biochemistry·A D CardinR L Jackson
Dec 1, 1993·The Journal of Cell Biology·R Bar-ShavitI Vlodavsky

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