PMID: 11310509Apr 20, 2001Paper

Specific localization of lysosomal aminopeptidases in type II alveolar epithelial cells of the rat lung

Archives of Histology and Cytology
Y YayoiY Uchiyama

Abstract

We previously demonstrated that lysosomal cysteine proteinases, cathepsins B, H, and L were localized in lysosomes of alveolar macrophages and bronchial epithelial cells in the rat lung, while cathepsin H, a typical aminopeptidase, was additionally distributed in lamellar bodies containing surfactant in type II alveolar epithelial cells (ISHII et al., 1991). The present immunohistochemical study further examined the localization of lysosomal aminopeptidases, cathepsin C, and tripeptidyl peptidase I (TPP-I) in the rat lung. Western blotting confirmed the presence of cathepsin C and TPP-I as active forms in the pulmonary tissue, showing 25 kD and 47 kD, respectively. Immunohisto/cytochemical observations demonstrated that positive staining for cathepsin C and TPP-I was more intensely localized in alveolar epithelial regions than in bronchial or bronchiolar epithelial cells. By double immunostaining using confocal laser microscopy, immunoreactivity for cathepsin H was found to be co-localized with that for cathepsin C or TPP-I in both type II cells and macrophages. Moreover, when doubly stained with anti-cathepsin C and ED2, single-positive type II cells could be clearly distinguished from double-positive macrophages in the alveol...Continue Reading

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Citations

Jan 7, 2004·Peptides·Elaine Gasparello-ClementePaulo Flávio Silveira
May 8, 2004·American Journal of Respiratory Cell and Molecular Biology·Ming JinHaifeng M Wu
Aug 18, 2006·Biological Chemistry·Gilles LalmanachVirginie Hervé-Grépinet
Oct 23, 2002·Archives of Histology and Cytology·Masato KoikeYasuo Uchiyama
Sep 24, 2002·Seminars in Cell & Developmental Biology·Timothy E WeaverMildred Stahlman
May 2, 2009·Toxicology in Vitro : an International Journal Published in Association with BIBRA·M Lynn Berndt-WeisCarole Yauk
Dec 16, 2005·Biology of the Cell·Carole Serveau-AvesqueGilles Lalmanach
Oct 3, 2012·Biochimica Et Biophysica Acta·Marianna Agassandian, Rama K Mallampalli
Oct 27, 2016·Cell and Tissue Research·Elena Lopez-RodriguezAnna Serrano-Mollar
Apr 28, 2004·Archives of Biochemistry and Biophysics·Kenji AkasakiJ Thomas August
May 15, 2007·The Journal of Biological Chemistry·Jining LüWellington V Cardoso
Feb 10, 2004·The Journal of Biological Chemistry·Takayuki UenoTimothy E Weaver
Mar 4, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Giuliana FerranteStefania La Grutta

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