PMID: 2974067Dec 1, 1988Paper

Specific lysis of allogeneic cells after activation of CD3- lymphocytes in mixed lymphocyte culture

The Journal of Experimental Medicine
E CicconeL Moretta

Abstract

Human CD3- lymphocyte populations were obtained by treating peripheral blood lymphocytes with mAbs directed to CD3, CD4, and CD8 surface antigens. The resulting populations were cultured with irradiated allogeneic cells; at day 4, 100 U/ml IL-2 were added and cultures continued for an additional 10 d. The resulting populations were CD3-CD2+CD7+ and displayed cytolytic activity against PHA-induced blast cells bearing the stimulating alloantigens but not against autologous or unrelated allogeneic blast cells. When CD3- populations were cultured with irradiated autologous cells, no cytolytic activity could be detected either against autologous or allogeneic blast cells. On the other hand, K562 target cells were lysed by both MLC-derived CD3- cell populations regardless of the origin (autologous or allogeneic) of the stimulating cells. CD3- clones were further derived from MLC-stimulated CD3- populations. These clones displayed a cytolytic pattern similar to the original MLC populations as only specific PHA blasts could be lysed. These clones did not express detectable surface TCR-alpha/beta or -gamma/delta molecules and lacked productive mRNA for TCR alpha and beta chains, while small amounts of TCR-gamma mRNA were detectable in o...Continue Reading

Citations

Jan 1, 1994·International Journal of Clinical & Laboratory Research·L MorettaA Moretta
Feb 1, 1992·Immunology Letters·E CicconeL Moretta
May 1, 1994·Immunology Letters·L MorettaA Moretta
Aug 1, 1992·Immunology Today·L MorettaK Kärre
Jan 1, 1989·Current Opinion in Immunology·S FerriniL Moretta
Feb 1, 1992·Current Opinion in Immunology·L L Lanier, J H Phillips
Jun 12, 2003·Developmental and Comparative Immunology·Linling ShenNorman W Miller
Aug 1, 1997·Molecular Immunology·L Jaso-FriedmannD L Evans
Jul 8, 2000·International Journal of Immunopharmacology·M L Salem, M S Hossain
Feb 1, 1996·Current Opinion in Immunology·M Colonna
Jun 1, 1996·Current Opinion in Immunology·D H Raulet
Dec 31, 2002·Microbes and Infection·Lorenzo MorettaAlessandro Moretta
Aug 30, 2002·Trends in Immunology·Andrea VelardiLorenzo Moretta
Nov 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·T SpiesJ L Strominger
Sep 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·J R BenderE Engleman
Dec 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·E CicconeA Moretta
Sep 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·M ColonnaO Viale
Dec 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·M ColonnaJ L Strominger
Mar 1, 1995·The Journal of Cell Biology·S PfauJ R Bender
Aug 1, 1990·The Journal of Experimental Medicine·N SuzukiE G Engleman
Apr 1, 1993·The Journal of Experimental Medicine·G BelloneG Trinchieri
Jan 1, 1994·Cancer Biotherapy·G Pawelec
Apr 27, 1997·Transplantation·E PeterssonG Hedlund
Oct 18, 2001·Current Opinion in Hematology·L RuggeriA Velardi
Jan 1, 1996·Annual Review of Immunology·A MorettaL Moretta

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.