PMID: 9525618Apr 3, 1998Paper

Specific methylation patterns in two control regions of Epstein-Barr virus latency: the LMP-1-coding upstream regulatory region and an origin of DNA replication (oriP)

Journal of Virology
K I FalkI Ernberg

Abstract

The Epstein-Barr virus (EBV) can establish at least four different forms of latent infection. Previously, we have shown that the level of methylation of the EBV genome varies, depending on the form of latency. The methylation status of CpGs was analyzed by the bisulfite genomic sequencing technique in four different cell types representing different forms of latency. The dyad symmetry element of the origin of replication (oriP) region and the latent membrane protein 1 (LMP-1) regulatory sequence (LRS) were studied. The dyad symmetry element has four binding sites for EBNA-1. In a cell with type I latency, a region upstream of the dyad symmetry element was highly methylated, whereas the dyad symmetry element was unmethylated in the EBNA-1-binding region. The LRS was extensively methylated in the LMP-1-negative cell line Rael, in contrast to a LMP-1-expressing nasopharyngeal carcinoma tumor (NPC C15), which was almost completely unmethylated. The methylation pattern of LRS in type I and type III Burkitt lymphoma cells of similar parental origins confirmed that demethylation of some regions takes place upon phenotypic drift.

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Citations

Jun 17, 2005·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Ildikó Györy, Janos Minarovits
Aug 15, 2009·PLoS Pathogens·Roland HutzingerAlexander Hüttenhofer
Mar 16, 2012·Annals of the New York Academy of Sciences·Ingemar ErnbergTomas J Ekström
Oct 28, 2004·Journal of Virology·Charles M Chau, Paul M Lieberman
Jan 10, 2018·Expert Review of Vaccines·Július RajčániSusan Szathmary
Aug 24, 2010·PLoS Pathogens·Italo TemperaPaul M Lieberman
Dec 25, 2010·Acta Microbiologica Et Immunologica Hungarica·J RajčániS Szathmary
Apr 20, 2017·Journal of Oncology·Shyam Singh, Hem Chandra Jha

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