Specific microRNAs regulate heat stress responses in Caenorhabditis elegans

Scientific Reports
Camilla NehammerRoger Pocock

Abstract

The ability of animals to sense and respond to elevated temperature is essential for survival. Transcriptional control of the heat stress response has been much studied, whereas its posttranscriptional regulation by microRNAs (miRNAs) is not well understood. Here we analyzed the miRNA response to heat stress in Caenorhabditis elegans and show that a discrete subset of miRNAs is thermoregulated. Using in-depth phenotypic analyses of miRNA deletion mutant strains we reveal multiple developmental and post-developmental survival and behavioral functions for specific miRNAs during heat stress. We have identified additional functions for already known players (mir-71 and mir-239) as well as identifying mir-80 and the mir-229 mir-64-66 cluster as important regulators of the heat stress response in C. elegans. These findings uncover an additional layer of complexity to the regulation of stress signaling that enables animals to robustly respond to the changing environment.

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Citations

Dec 24, 2015·Frontiers in Medicine·Cristina Sorina CătanăIoana Berindan-Neagoe
May 1, 2016·In Vitro Cellular & Developmental Biology. Animal·Kun-Lin ChenHui-Xia Li
Apr 4, 2017·Molecular Ecology·Andrian P Gajigan, Cecilia Conaco
Aug 25, 2017·PloS One·Jessica BrunquellSandy D Westerheide
Jan 27, 2019·Genetics·Hina IftikharGinger E Carney
Aug 2, 2018·Médecine sciences : M/S·Rachel LitkeChantal Fradin
Oct 9, 2016·BMC Biology·Rafael C FerrazMarcelo A Mori
Aug 10, 2019·Nucleic Acids Research·William P SchreinerAmy E Pasquinelli
Aug 6, 2019·Developmental Cell·ChangHwan LeeJudith Kimble
Mar 24, 2018·Cell·David P Bartel
Aug 6, 2021·PLoS Genetics·Delaney C PagliusoAmy E Pasquinelli
Oct 12, 2021·Experimental Gerontology·Brian EvansAlexandre de Lencastre

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Methods Mentioned

BETA
environmental stress
PCR
transgenic
Assay
electrophoresis

Software Mentioned

PERL script
genEx
R
NormFinder
PicTar
GeNorm
miRDeep2

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