Jul 6, 2016

Specific miRNA Disease Biomarkers in Blood, Serum and Plasma: Challenges and Prospects

Molecular Diagnosis & Therapy
Christina BackesAndreas Keller

Abstract

Significant effort has been devoted to discovering microRNA (miRNA) disease biomarkers. In particular, miRNAs in whole blood or specific blood components are candidates for improving the diagnosis of diseases, including life-threatening pathologies. This review covers the challenges crucial for the translation of miRNAs in body fluids (circulating miRNAs) from a research setting into a clinical care scenario. First, we discuss the specificity of miRNA biomarkers for the diagnosis of a disease. While single miRNAs such as miR-20a, miR-21, miR-155, and miR-126 are frequently not disease specific, miRNA signatures that consist of a plurality of different miRNAs may help to improve differentiation between pathologies. Second, we discuss the degree of reproducibility and highlight selected validation studies. While single miRNA markers are often confirmed by independent studies, miRNA signatures are less frequently verified. Third, we address challenges to the profiling of miRNAs in high-throughput settings and we discuss the appropriateness of various analytical platforms and bioinformatics towards a clinical application of miRNAs. Finally, we shed light on the suitability of enriched miRNA sources, e.g. fractionation of body fluid...Continue Reading

  • References66
  • Citations25

References

  • References66
  • Citations25

Mentioned in this Paper

Exosomes
Biological Markers
Study
Whole Blood
Extracellular
MIR20A
MIRN21 microRNA, mouse
RNA, Small Temporal
Bio-Informatics
Vesicle

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