Specific Neisseria gonorrhoeae DNA-probes derived from ribosomal RNA.

Journal of General Microbiology
R RossauH Van Heuverswijn

Abstract

Eighteen sequences complementary to less-conserved regions within the 16S and 23S ribosomal ribonucleic acid (rRNA) of Neisseria gonorrhoeae were subcloned or chemically synthesized and used as probes in a dot-spot deoxyribonucleic acid (DNA): DNA hybridization format. Some of these probes exclusively detected Neisseria gonorrhoeae nucleic acid, whereas others also showed hybridization signals with nucleic acid from other bacterial species. Our results indicate that rRNA-derived DNA-probes can be used to differentiate between very closely related taxa without the use of Southern-blot analysis.

Citations

Mar 1, 1993·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·W M JandaJ M Stevens
Jan 1, 1991·International Journal of Food Microbiology·W E Hill, S P Keasler
May 1, 1992·Journal of Clinical Pathology·B S HoS I Egglestone

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