Specific nicking-closing activity of the initiator of replication protein RepB of plasmid pMV158 on supercoiled or single-stranded DNA.

The Journal of Biological Chemistry
M MoscosoM Espinosa


Asymmetric rolling circle replication of the promiscuous replicon pMV158 is initiated by the plasmid-encoded RepB protein. In vitro, purified RepB protein introduces a nick within the leading strand origin of replication by a nucleophylic attack on the phosphodiester bond at the dinucleotide GpA. Some changes within and around this dinucleotide were recognized by the protein. RepB nicked and closed supercoiled pMV158 DNA, having an optimum activity at 60 degrees C. We have imitated, in vitro, a process of rolling circle replication, since RepB was able to nick (initiation) and to covalently close (termination) single-stranded oligonucleotides containing the protein cleavage sequence. Covalent DNA-protein complexes were not found, indicating that RepB has unique features among plasmid-encoded proteins involved in rolling-circle replication or conjugative mobilization.


May 20, 1990·Journal of Molecular Biology·A G de la CampaM Espinosa
Nov 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·P NoirotR P Novick
Jun 1, 1989·Microbiological Reviews·A Gruss, S D Ehrlich
Jan 1, 1989·Annual Review of Microbiology·R P Novick
Sep 1, 1989·Journal of Bacteriology·S D Priebe, S A Lacks
Mar 1, 1987·Molecular & General Genetics : MGG·G del SolarM Espinosa
May 26, 1987·Nucleic Acids Research·R R Koepsel, S A Khan
Nov 29, 1974·Science·I R Lehman
Aug 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·S A KhanR P Novick
Apr 1, 1994·Journal of Bacteriology·A RasoolyR P Novick
Apr 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·W PansegrauE Lanka
Feb 1, 1993·Molecular & General Genetics : MGG·G del SolarM Espinosa


Jan 17, 2004·Biochimica Et Biophysica Acta·José A Ruiz-MasóG del Solar
Dec 24, 1997·Trends in Microbiology·A Rasooly, R S Rasooly
Aug 2, 2013·Proceedings of the National Academy of Sciences of the United States of America·María Victoria FranciaGabriel Moncalian
Jul 8, 1997·Proceedings of the National Academy of Sciences of the United States of America·E L ZechnerG Högenauer
Oct 7, 2016·Frontiers in Molecular Biosciences·José A Ruiz-MasóG del Solar
Dec 9, 2017·Frontiers in Microbiology·Stephen M KwongNeville Firth
Aug 1, 1995·FEMS Microbiology Letters·M EspinosaJ C Alonso
Mar 31, 2018·Frontiers in Molecular Biosciences·Fabián Lorenzo-DíazM Espinosa
Jun 10, 1998·Microbiology and Molecular Biology Reviews : MMBR·G del SolarR Díaz-Orejas
Jun 25, 2015·Microbiology Spectrum·José A Ruiz-MasóG del Solar
Dec 31, 1997·Microbiology and Molecular Biology Reviews : MMBR·S A Khan

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