Specific PCR identification of Pasteurella multocida based on putative transcriptional regulator genes

Journal of Microbiological Methods
D LiuF W Austin

Abstract

Pasteurella multocida is an important animal pathogen that may also infect humans through animal bites and scratches. After comparison of transcriptional regulator gene sequences from the P. multocida genome with other DNA sequences at GenBank, we identified two genes (i.e., Pm0762 and Pm1231) uniquely present in P. multocida. By using oligonucleotide primers (Pm0762F/R and Pm1231F/R) designed from these genes in PCR, it was found that specific DNA products of expected sizes were obtained with genomic DNA from P. multocida only, but not from other bacteria. These results indicated that the putative transcriptional regulator genes Pm0762 and Pm1231 are species-specific, and that the PCR methods targeting these genes provide a useful means of rapidly and precisely identifying P. multocida from other bacteria. Further elucidation of the roles and functions of these putative transcriptional regulator genes (Pm0762 and Pm1231) and their protein products may help provide valuable insight into the molecular mechanism of P. multocida virulence and pathogenicity.

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Citations

Oct 4, 2007·Diagnostic Microbiology and Infectious Disease·Zhan-Min LiuFeng Pan
Dec 7, 2007·Veterinary Microbiology·Francis DzivaHenrik Christensen
Mar 10, 2007·Journal of Microbiological Methods·B G CorneyP J Blackall
May 22, 2009·Journal of Applied Microbiology·C MaroisM Kobisch
Jan 1, 2014·FEMS Microbiology Letters·Ciro C RossiDenise M S Bazzolli
Jul 12, 2014·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Colin J BellHywel J Ball
Jan 12, 2017·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Gerard M MurrayJoseph P Cassidy
Sep 22, 2018·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·John D LoyBing Wang
Apr 11, 2006·Canadian Journal of Microbiology·Dongyou LiuLanny W Pace
May 13, 2017·Research in Veterinary Science·V TocquevilleC Marois-Créhan

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