Specific PKC isoforms regulate blastocoel formation during mouse preimplantation development

Developmental Biology
Judith EckertTom P Fleming

Abstract

During early mammalian development, blastocyst morphogenesis is achieved by epithelial differentiation of trophectoderm (TE) and its segregation from the inner cell mass (ICM). Two major interrelated features of TE differentiation required for blastocoel formation include intercellular junction biogenesis and a directed ion transport system, mediated by Na+/K+ ATPase. We have examined the relative contribution of intercellular signalling mediated by protein kinase C (PKC) and gap junctional communication in TE differentiation and blastocyst cavitation. The distribution pattern of four (delta, theta, iota/lambda, zeta) PKC isoforms and PKCmicro/PKD1 showed partial colocalisation with the tight junction marker ZO-1alpha+ in TE and all four PKCs (delta, theta, iota/lambda, zeta) showed distinct TE/ICM staining patterns (predominantly at the cell membrane within the TE and cytoplasmic within the ICM), indicating their potential contribution to TE differentiation and blastocyst morphogenesis. Specific inhibition of PKCdelta and zeta activity significantly delayed blastocyst formation. Although modulation of these PKC isoforms failed to influence the already established programme of epithelial junctional differentiation within the TE...Continue Reading

References

Jan 1, 1988·Annual Review of Cell Biology·T P Fleming, M H Johnson
Jun 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·G M KidderJ McKeon
Jun 20, 1995·Proceedings of the National Academy of Sciences of the United States of America·R O Stuart, S K Nigam
Aug 9, 1996·Science·M LeitgesA Tarakhovsky
Feb 1, 1996·The Journal of Membrane Biology·V Dodane, B Kachar
Apr 1, 1997·Molecular Reproduction and Development·G I GallicanoD G Capco
Dec 24, 1997·Annals of the New York Academy of Sciences·M S FeschenkoK J Sweadner
Apr 16, 1998·The Journal of Biological Chemistry·Z HanR V Patil
May 16, 1998·The Journal of Biological Chemistry·A V ChibalinA M Bertorello
May 28, 1998·The Journal of Biological Chemistry·T ToyofukuM Tada
Jun 26, 1998·The Biochemical Journal·H Mellor, P J Parker
Oct 14, 1998·International Review of Cytology·K Matter, M S Balda
Oct 27, 1998·Nature Biotechnology·M C Souroujon, D Mochly-Rosen
Apr 1, 1999·Annual Review of Physiology·T W White, D L Paul
Apr 20, 1999·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·M Csukai, D Mochly-Rosen
Sep 2, 1999·Molecular Reproduction and Development·C M Pauken, D G Capco
Sep 24, 1999·Biology of Reproduction·R BoniB Dale
Oct 27, 1999·Proceedings of the National Academy of Sciences of the United States of America·C H ChenD Mochly-Rosen
Feb 23, 2000·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·S Jaken, P J Parker

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Citations

May 10, 2011·BMC Genomics·Luiza F AndradeGuilherme Oliveira
Apr 28, 2009·International Journal for Parasitology·Marthe H R LudtmannAnthony J Walker
Dec 25, 2007·Molecular and Cellular Endocrinology·Nicolas DardSophie Louvet-Vallée
Jul 24, 2015·Molecular Reproduction and Development·Wei MaMaria M Viveiros
Mar 15, 2008·Biochimica Et Biophysica Acta·Judith J Eckert, Tom P Fleming
Nov 22, 2007·Biochimica Et Biophysica Acta·Marcelino CereijidoIsabel Larre
Oct 24, 2007·Biochimica Et Biophysica Acta·Lorenza González-MariscalDavid Chamorro
Sep 30, 2015·The Journal of Cell Biology·Sylvain BessonnardDaniel B Constam
Dec 7, 2013·International Journal of Cancer. Journal International Du Cancer·Hannah R WardillJoanne M Bowen
Aug 26, 2006·Gene Expression Patterns : GEP·Judit KovacMichael Leitges
Jun 14, 2005·Developmental Biology·Stéphanie VinotSophie Louvet-Vallée
Jun 18, 2016·Developmental Biology·In Kyoung MahFrancesca V Mariani
Jun 15, 2006·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Yojiro YamanakaJanet Rossant
Sep 26, 2006·Molecular Reproduction and Development·Xiang-Shun CuiNam-Hyung Kim
Nov 1, 2016·Journal of the Royal Society, Interface·Sebastian V HadjiantoniouAndrew E Pelling
Aug 15, 2014·Reproduction, Fertility, and Development·Qi-En YangAlan D Ealy
Aug 9, 2012·Physical Biology·Efrat Oron, Natalia Ivanova
Apr 16, 2015·Reproductive Medicine and Biology·Agnieszka Jedrusik
Sep 7, 2012·Reproduction, Fertility, and Development·Shao-Chen SunNam-Hyung Kim
Jul 24, 2020·Development·Berenika Płusa, Anna Piliszek

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