Specific polyclonal-based immunoassays for sulfathiazole

Analytical and Bioanalytical Chemistry
Nuria Pastor-NavarroRosa Puchades

Abstract

A highly sensitive and specific enzyme-linked immunosorbent assay has been developed for detection of sulfathiazole (STZ, 4-amino- N-thiazol-2-yl-benzenesulfonamide). A set of haptens was synthesized in order to produce polyclonal antibodies against sulfonamides. Two ELISA formats (antibody-coated and conjugate-coated) were also investigated using all the serum/coating conjugate combinations that showed specific recognition. The developed ELISA succeeded in detection of STZ at concentrations as low as 0.03 ng mL(-1) over a measurable range of 0.12-6.71 ng mL(-1). Selectivity studies have demonstrated that other sulfonamides do not interfere significantly (<10%) with analysis of STZ by this immunochemical technique. Analysis of spiked bee honey samples by the developed ELISA method showed recoveries were good. The selectivity and sensitivity (IC(50)=1.6 ng mL(-1)) make it a suitable screening method for determination of low levels of STZ in food samples.

Citations

Jul 2, 2009·Analytical and Bioanalytical Chemistry·Nuria Pastor-NavarroRosa Puchades
May 6, 2011·Chemical Reviews·Naomi J HalasPeter Nordlander
Jul 11, 2012·Journal of Medicinal Chemistry·Katia D'AmbrosioClaudiu T Supuran
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Aug 1, 2009·Journal of Immunological Methods·Hongyan Zhang, Shuo Wang
Mar 28, 2007·Analytica Chimica Acta·Nuria Pastor-NavarroRosa Puchades
May 14, 2009·Journal of Environmental Monitoring : JEM·Nuria Pastor-NavarroRosa Puchades
May 13, 2011·Organic & Biomolecular Chemistry·Celia Suárez-PantaleónAntonio Abad-Fuentes
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Jun 22, 2006·Journal of Agricultural and Food Chemistry·Hongyan ZhangShuo Wang

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