PMID: 9160248May 1, 1997Paper

Specific proteolytic cleavage of the myristoylated alanine-rich C kinase substrate between Asn 147 and Glu 148 also occurs in brain

Journal of Neuroscience Research
S ManentiJ M Darbon

Abstract

The myristoylated alanine-rich C kinase substrate (MARCKS) is a major ubiquitous substrate of protein kinase C. The expression of the protein is regulated during cell cycle progression and cell proliferation. Specific proteolytic cleavage of the protein between Asn 147 and Glu 148 was described recently in cultured cells, and the corresponding proteolytic activity was observed in various tissue extracts except for brain. We purified a 40 kDa fragment of MARCKS from bovine brain that we characterized as the C-terminal specific fragment found in other tissues. The identification of the fragment was achieved by in vitro phosphorylation by protein kinase C, calcium-dependent interaction with calmodulin, mass spectrometric analysis, and N-terminal sequencing. These data suggest that specific proteolytic cleavage of MARCKS also occurs in brain and may be a general mechanism of down-regulation of the protein.

References

Sep 15, 1989·The Biochemical Journal·K K WangB D Roufogalis
May 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·K A AlbertP Greengard
Feb 14, 1995·Proceedings of the National Academy of Sciences of the United States of America·D J StumpoP J Blackshear

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Citations

Jun 20, 2012·Pulmonary Pharmacology & Therapeutics·W Randall LampeKenneth B Adler
Oct 13, 2000·American Journal of Physiology. Cell Physiology·Y ZhaoH W Davis
Mar 29, 2000·The International Journal of Biochemistry & Cell Biology·J J Ramsden

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