Specific radioimmunoassays for IL 1 alpha and IL 1 beta in plasma at physiological and acidic pH: determination of immunoreactive forms by gel filtration and radioligand binding studies

Cytokine
S J CapperT H Mander

Abstract

We have developed specific radioimmunoassays for interleukin 1 alpha (IL 1 alpha) and interleukin 1 beta (IL 1 beta) and applied these successfully to the measurement of interleukin 1 (IL 1) in neat plasma. Further characterization of the plasma immunoreactive forms of IL 1 was done using Sephadex G-75 chromatography and TSKG2000 high performance gel permeation chromatography. This revealed the immunoreactivity to be associated with a high molecular weight fraction for both IL 1 alpha and IL 1 beta. Incubation of plasma with iodinated IL 1 alpha and beta showed that there was a time-dependent association of tracer with the high molecular weight fraction and that this was predominantly with IL 1 beta. The activity was displaceable with unlabeled IL 1 beta, which together with the chromatography results, suggested that IL 1 beta is protein-bound in plasma. Furthermore, we have shown that under acid conditions both tracer and endogenous IL 1 beta immunoreactivity migrate as a low (17 kD) molecular weight fraction. This suggests that dissociation from a high molecular weight binder has occurred. Acid treatment of plasma raised the immunoreactive IL 1 beta level, but had no effect on IL 1 alpha levels, confirming the specificity of ...Continue Reading

References

Jan 1, 1990·Immunology Today·F S di Giovine, G W Duff
Apr 1, 1989·Scandinavian Journal of Immunology·M SvensonK Bendtzen
Feb 1, 1986·Immunology Today·J J OppenheimS K Durum

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Citations

Apr 1, 1991·Current Opinion in Immunology·M P Beckmann, P J Morrissey
Jan 26, 1999·Annals of the New York Academy of Sciences·R R GonzalezW F Allison
Nov 1, 1993·The Journal of Clinical Investigation·M SvensonK Bendtzen
Oct 8, 2005·The International Journal of Artificial Organs·J V Di Carlo, S R Alexander

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