Specific receptor binding of renin on human mesangial cells in culture increases plasminogen activator inhibitor-1 antigen

Kidney International
Genevieve NguyenJean-Daniel Sraer

Abstract

Some proteases possess a membrane receptor that focalizes their proteolytic activity on the cell surface and may mediate a proliferative effect, such as urokinase on glomerular epithelial cells. Since some hypertensive states are associated with high concentrations of renin and proliferation of arteriolar smooth muscle cells, we asked whether renin, an aspartyl-protease, would bind to mesangial cells that are smooth-muscle derived cells, which would induce their proliferation. The binding of 125I labeled recombinant human renin (125I-R) was studied on human primary mesangial cells and mesangial cells immortalized by transfection with SV40-T antigen. At 37 degrees C, the binding of 125I-R was time dependent and reached a plateau after two hours. 125I-R was found to bind in a saturable and specific manner with a Kd = 0.4 nM and 1 nM and 8,000 and 2,000 binding sites/cell, for primary and immortalized cells, respectively. When binding experiments were performed in the presence RO 42-5892, a synthetic inhibitor of renin, RO 42-5892 could inhibit the specific binding of labeled renin only at concentrations 1,000 times superior to the IC 50, indicating that the renin-mesangial receptor interaction did not depend on the active site of...Continue Reading

References

Feb 28, 1978·Biochemical and Biophysical Research Communications·P J Fraker, J C Speck
Jun 1, 1992·Kidney International·D F GreenJ J Bourgoignie
Jul 1, 1991·Hypertension·W FischliQ Branca
Nov 1, 1990·Kidney International·L M VillamedianaJ D Sraer
Jan 1, 1986·Journal of Cardiovascular Pharmacology·V J Dzau, J Kreisberg
Oct 1, 1989·Physiological Reviews·P MenéM J Dunn
Jan 1, 1987·The Journal of Clinical Investigation·D J Campbell
Sep 1, 1983·Hypertension·M LoudonJ D Swales
Nov 1, 1993·Circulation Research·N TomitaT Ogihara
Jan 1, 1993·Hypertension·P W AndersonW A Hsueh
Jan 1, 1996·Kidney International·J D SraerE Rondeau

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Citations

Mar 7, 2008·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Geneviève Nguyen, Aurélie Contrepas
Mar 13, 2008·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Jan H SchefeHeiko Funke-Kaiser
Mar 7, 2012·Cardiovascular Drugs and Therapy·Michel Azizi, Joël Ménard
Mar 11, 2004·Current Hypertension Reports·Geneviève NguyenJean-Daniel Sraer
Apr 20, 2007·Current Hypertension Reports·Yufeng HuangNancy A Noble
Oct 22, 2008·Current Hypertension Reports·Tadashi Inagami, Atsuhiro Ichihara
Jul 16, 2008·Current Hypertension Reports·Brian K Dockery, John D Bisognano
Sep 12, 2000·Current Hypertension Reports·A B Fogo
Sep 13, 2013·Peptides·Hajime YamamotoKazuhiro Takahashi
Aug 2, 2003·Trends in Endocrinology and Metabolism : TEM·Robert M Carey, Helmy M Siragy
Apr 5, 2003·The International Journal of Biochemistry & Cell Biology·A H Jan Danser
Mar 6, 2010·Hypertension Research : Official Journal of the Japanese Society of Hypertension·David L Feldman
May 5, 2010·Nature Reviews. Nephrology·Anne-Emilie Declèves, Kumar Sharma
Feb 4, 2010·Journal de la Société de biologie·Diane BracquartGeneviève Nguyen
Aug 18, 2006·Current Opinion in Nephrology and Hypertension·Michel Azizi
May 13, 2009·American Journal of Therapeutics·Jon BergsetJohn D Bisognano
Feb 13, 2007·Current Opinion in Nephrology and Hypertension·Geneviève Nguyen
Nov 26, 2010·Current Opinion in Nephrology and Hypertension·Jennifer L Wilkinson-BerkaKatrina J Binger
Jun 26, 2007·International Journal of Clinical Practice·J NussbergerM F Prescott
Jun 6, 2009·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Eric Lazartigues
Apr 27, 2012·American Journal of Physiology. Renal Physiology·Jiandong ZhangYufeng Huang
Dec 17, 2009·American Journal of Physiology. Renal Physiology·Adam Whaley-ConnellJames R Sowers
Jun 5, 2002·The Journal of Clinical Investigation·Genevieve NguyenJean-Daniel Sraer
Apr 19, 2011·World Journal of Cardiology·Gaurav Alreja, Jacob Joseph
Apr 4, 2008·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·M Ian Phillips, Edilamar Menezes de Oliveira
Nov 15, 2002·Journal of Molecular and Cellular Cardiology·A H Jan Danser, Jasper J Saris
Nov 15, 2002·Journal of Molecular and Cellular Cardiology·Julia L CookRichard N Re
Jan 15, 2013·International Journal of Hypertension·Wencheng LiYumei Feng
Apr 9, 2013·Clinical and Experimental Nephrology·Kazu HamadaYoshio Terada
Aug 3, 2000·Gynecological Endocrinology : the Official Journal of the International Society of Gynecological Endocrinology·W U AtiomoA G Prentice
Sep 23, 2006·Expert Opinion on Investigational Drugs·Eoin O'Brien
Mar 1, 2006·Expert Opinion on Pharmacotherapy·Jens GaedekeHarm Peters
Sep 4, 2008·Expert Opinion on Emerging Drugs·Helmy SiragyDavid C Lieb
Jul 10, 2012·American Journal of Therapeutics·Alex KorniyenkoGagangeet Sandhu
Sep 25, 2014·The American Journal of the Medical Sciences·Alexis A GonzalezMinolfa C Prieto

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