PMID: 6972041Feb 1, 1981Paper

Specific receptors for phorbol esters in lymphoid cell populations: role in enhanced production of T-cell growth factor

Proceedings of the National Academy of Sciences of the United States of America
J J SandoJ J Farrar

Abstract

Phorbol ester tumor promoters act synergistically with concanavalin A to cause production of T-cell growth factor by normal human peripheral blood lymphocytes. A specific, saturable, binding component which may mediate the phorbol ester effect has been identified by using [20-3H]phorbol 12,13-dibutyrate in a whole-cell binding assay. Specific binding is maximal with 5 min at 37 or 23 degrees C but the level of bound ligand rapidly decreases to about 50% within 1 hr. At 4 degrees C, 2 hr are required to reach maximal binding, and the binding is stable for at least 20 hr. Binding is reversible at 37 and 4 degrees C with time courses similar to those for initial binding at the respective temperatures. Saturation of the specific binding occurs at a concentration (approximately 30 nM) consistent with that producing maximal T-cell growth factor activity. Scatchard analysis of the binding after 30 min at 37 degrees C demonstrates a lower Kd (9 nM) than that determined after 2 hr at 4 degrees C (22 nM). The median number of sites per cell for six donors was 2 X 10(5) (range, 1.3-4 X 10(5). Other tumor-promoting phorbol esters compete for [20-3H]phorbol 12,13-dibutyrate binding in approximate proportion to their activity in stimulating ...Continue Reading

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Citations

May 1, 1984·Journal of Clinical Immunology·J OkamuraE W Gelfand
Jan 1, 1991·Neurochemical Research·G Shanker, H J Baker
Jan 1, 1990·International Journal of Immunopharmacology·J FaveroJ W Parker
May 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·R A CooperA L Kuo
Jul 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·K L LeachP M Blumberg
May 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·J J Sando, M C Young
May 1, 1992·The Journal of Experimental Medicine·R SchreckP A Baeuerle
Jun 1, 1995·Photodermatology, Photoimmunology & Photomedicine·D LeszczynskiK Servomaa
Oct 1, 1982·The Journal of Clinical Investigation·B J Goodwin, J B Weinberg
Mar 1, 1983·Clinical Immunology and Immunopathology·I Sugawara, S Ishizaka
Feb 1, 1988·The Journal of protozoology·J I RavdinP H Schlesinger
Jul 1, 1984·The British Journal of Dermatology·D A Chambers, R L Cohen
Nov 1, 1982·The British Journal of Dermatology·D M MacDonald
May 1, 1985·British Journal of Haematology·D A PolanskyD M Miller
Sep 15, 1987·Biochemical and Biophysical Research Communications·B Calabretta, W E Mercer
Aug 1, 1985·Biochemical Pharmacology·A Gescher
Apr 14, 1987·Biochemical and Biophysical Research Communications·A S KraftR L Berkow
Mar 1, 1988·Human Toxicology·R S DewdneyD Anderson
Sep 1, 1990·Scandinavian Journal of Immunology·T A Springer
Apr 1, 1987·Scandinavian Journal of Immunology·G GromoF H Bach

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