Specific recognition of A/G and A/7,8-dihydro-8-oxoguanine (8-oxoG) mismatches by Escherichia coli MutY: removal of the C-terminal domain preferentially affects A/8-oxoG recognition

Biochemistry
A GogosA L Lu

Abstract

Escherichia coli MutY is a 39 kDa adenine DNA glycosylase and 3' apurinic/apyrimidinic (AP) lyase that is active on DNA substrates containing A/G, A/C, or A/8-oxoG mismatches. 8-oxoG (7,8-dihydro-8-oxoguanine or GO) is a major stable product of oxidative damage, and A/GO mismatches may be particularly important biological substrates for MutY. Proteolytic digestion of MutY using thermolysin was found to produce two relatively stable fragments of 25 and 12 kDa. The 25 kDa fragment begins at the N terminus of MutY and spans the region homologous with E. coli endonuclease III, a DNA glycosylase/AP lyase that repairs oxidatively damaged pyrimidines. The 12 kDa fragment, which consists of much of the rest of MutY, had no detectable activity. The purified 25 kDa fragment (M25) had nearly wild-type binding and cleavage activities with A/G-mismatched substrates. Binding to A/GO-mismatched DNA, however, was dramatically reduced in M25 compared to that in intact protein. Borohydride-dependent enzyme-DNA cross-linking, which is a hallmark of the reaction of several DNA glycosylases that possess concomitant AP lyase activity, was also substantially reduced when M25 was allowed to react with A/GO-mismatched DNA. The significant differences i...Continue Reading

Citations

Jun 12, 2002·Free Radical Biology & Medicine·Dmitry O Zharkov, Arthur P Grollman
Jun 15, 2000·Journal of Bacteriology·M M Samrakandi, F Pasta
Jul 20, 1999·Annual Review of Biophysics and Biomolecular Structure·C D MolJ A Tainer
Oct 16, 2003·Proceedings of the National Academy of Sciences of the United States of America·Elizabeth M BoonJacqueline K Barton
Nov 28, 2015·The FEBS Journal·Carlos H Trasviña-ArenasLuis G Brieba
Mar 20, 2013·Mutation Research·Filomena MazzeiMargherita Bignami
Jun 30, 2007·Journal of Inorganic Biochemistry·Amie K BoalJacqueline K Barton
Nov 10, 2004·DNA Repair·Mary Ann Pope, Sheila S David
Mar 17, 2015·Mutation Research·Ana Helena Sales de OliveiraLucymara Fassarella Agnez-Lima
Apr 20, 2016·Proceedings of the National Academy of Sciences of the United States of America·Hongan LongMichael Lynch
Oct 3, 1999·The Journal of Biological Chemistry·P M WrightA L Lu
Oct 2, 2009·Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics·Ye-Fei WangCheng-Bu Liu
Jul 8, 2000·The Journal of Biological Chemistry·D O ZharkovA P Grollman
Nov 21, 2002·The Journal of Biological Chemistry·Isaac WongJacqueline A Wirz
Oct 6, 2005·The Biochemical Journal·A-Lien LuXianghong Li
Sep 8, 2009·The Journal of Physical Chemistry. a·Ye-Fei WangCheng-Bu Liu

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