Specific regulation of PRMT1 expression by PIAS1 and RKIP in BEAS-2B epithelia cells and HFL-1 fibroblasts in lung inflammation

Scientific Reports
Li LiuShemin Lu

Abstract

Protein arginine methyltransferase 1 (PRMT1) catalyzes methylation of histones and other cellular proteins, and thus regulates gene transcription and protein activity. In antigen-induced pulmonary inflammation (AIPI) PRMT1 was up-regulated in the epithelium, while in chronic AIPI, increased PRMT1 shifted to fibroblasts. In this study we investigated the cell type specific regulatory mechanism of PRMT1. Epithelial cells and fibroblasts were stimulated with IL-4 or IL-1β. Gene and protein expression were determined by RT-qPCR, immunohistochemistry staining and Western blotting. Signaling pathway inhibitors, siRNAs and shRNA were used to determine the regulatory mechanism of PRMT1. The results showed that IL-4 up-regulated PRMT1 through STAT6 signaling in epithelial cells, while IL-1β regulated PRMT1 through NF-κB in fibroblasts. The NF-kB inhibitor protein RKIP was highly expressed in epithelial cells and blocked IL-1β induced PRMT1 up-regulation; while the STAT6 inhibitor protein PIAS1 was expressed in fibroblasts and suppressed IL-4 induced PRMT1 expression. Furthermore, IL-4 stimulated epithelial cells to release IL-1β which up-regulated PRMT1 expression in fibroblasts. In conclusion, the inhibitor proteins RKIP and PIAS1 regu...Continue Reading

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Citations

Jan 13, 2017·Epigenetics : Official Journal of the DNA Methylation Society·Suzanne AcklooSusanne Müller
Nov 3, 2017·American Journal of Physiology. Cell Physiology·Derek W StouthVladimir Ljubicic
Nov 27, 2019·Biomolecules·Maria Gabriela-FreitasOlga Martinho
Jan 26, 2020·International Journal of Molecular Sciences·Lei FangMichael Roth
Feb 18, 2020·The Journal of Toxicological Sciences·Chenghui LengXiaojing Feng
Nov 27, 2020·The Journal of Immunology : Official Journal of the American Association of Immunologists·Weiqi ZhaiMichael Roth
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Mar 27, 2021·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Zhi ZhangFanhong Zeng
Jul 11, 2021·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·Stephany Sánchez-OvandoPeter A B Wark

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Methods Mentioned

BETA
co-IP
transfection
FCS
biopsies
PCR
co-immunoprecipitation

Software Mentioned

Genomatix
ImageJ

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