PMID: 9188103Mar 1, 1997Paper

Specific release of membrane-bound annexin II and cortical cytoskeletal elements by sequestration of membrane cholesterol

Molecular Biology of the Cell
T HarderJean Gruenberg

Abstract

Annexin II is an abundant protein which is present in the cytosol and on the cytoplasmic face of plasma membrane and early endosomes. It is generally believed that this association occurs via Ca(2+)-dependent binding to lipids, a mechanism typical for the annexin protein family. Although previous studies have shown that annexin II is involved in early endosome dynamics and organization, the precise biological role of the protein is unknown. In this study, we found that approximately 50% of the total cellular annexin was associated with membranes in a Ca(2+)-independent manner. This binding was extremely tight, since it resisted high salt and, to some extent, high pH treatments. We found, however, that membrane-associated annexin II could be quantitatively released by low concentrations of the cholesterol-sequestering agents filipin and digitonin. Both treatments released an identical and limited set of proteins but had no effects on other membrane-associated proteins. Among the released proteins, we identified, in addition to annexin II itself, the cortical cytoskeletal proteins alpha-actinin, ezrin and moesin, and membrane-associated actin. Our biochemical and immunological observations indicate that these proteins are part of...Continue Reading

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Citations

Apr 4, 1998·Electrophoresis·M RojoJ Gruenberg
Apr 4, 1998·Electrophoresis·F G van der Goot
Apr 5, 2008·Pflügers Archiv : European journal of physiology·Grazia TammaGiovanna Valenti
Aug 1, 1997·Current Opinion in Cell Biology·T Harder, K Simons
Dec 9, 2000·Biochimica Et Biophysica Acta·C J Fielding, P E Fielding
Jun 2, 2005·Nature Reviews. Molecular Cell Biology·Volker GerkeStephen E Moss
Apr 24, 2009·The Journal of Biological Chemistry·Katia MonastyrskayaAnnette Draeger
Sep 29, 2011·The Journal of Biological Chemistry·Atoosa RezvanpourGary S Shaw
Dec 10, 1999·The Journal of Experimental Medicine·P C ChengS K Pierce
Jan 22, 2004·The Journal of Experimental Medicine·Clare JollyQuentin J Sattentau
Dec 31, 2003·The Journal of General Physiology·Victor G RomanenkoIrena Levitan
Sep 5, 2008·Molecular Biology of the Cell·Thomas FalguièresJean Gruenberg
Dec 15, 2010·Molecular Biology of the Cell·Dafne ChirivinoMonique Arpin
Dec 28, 2007·Molecular and Cellular Biology·Stéphanie HeyraudDanielle Gulino-Debrac
Oct 6, 1998·Annual Review of Biochemistry·R G Anderson
Oct 14, 2000·Annual Review of Cell and Developmental Biology·A BretscherD Reczek
Jan 28, 2006·American Journal of Respiratory Cell and Molecular Biology·Narendranath Reddy ChintagariLin Liu
Jul 13, 2011·Journal of Lipid Research·Amol KavishwarAnna Moore
Apr 27, 2001·Biochemical and Biophysical Research Communications·J Ayala-Sanmartin
May 30, 2013·Cellular and Molecular Life Sciences : CMLS·Nina QuiskampUrsula Rescher
Jun 16, 2004·Proceedings of the National Academy of Sciences of the United States of America·Huiren ZhaoJerrold R Turner
Feb 21, 2002·Proceedings of the National Academy of Sciences of the United States of America·Michelangelo FotiJean-Louis Carpentier
Jun 13, 2002·Proceedings of the National Academy of Sciences of the United States of America·Timothy E HawkinsStephen E Moss
Apr 22, 2009·Cellular and Molecular Life Sciences : CMLS·Katia MonastyrskayaAnnette Draeger
Dec 14, 2011·Cellular and Molecular Life Sciences : CMLS·Magdalena DomonJoanna Bandorowicz-Pikula
Dec 31, 2003·The Journal of Cell Biology·Christelle BenaudJacques Baudier
Sep 7, 2000·The Journal of Cell Biology·E B Babiychuk, A Draeger
Sep 23, 1998·The Journal of Cell Biology·F LafontK Simons
Nov 11, 2009·Archives of Biochemistry and Biophysics·Malgorzata E SztolsztenerJoanna Bandorowicz-Pikula

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