Specific role of manganese and magnesium on RNA synthesis in rabbit bone marrow erythroid cell nuclei

Biological Trace Element Research
M K Song, J A Hunt

Abstract

Specific roles of manganese (Mn) and magnesium (Mg) on the activities of DNA-dependent RNA polymerases I and II isolated from rabbit bone marrow erythroid cell nuclei were investigated. Three main polymerases were separated from the cell nuclei. When RNA polymerase I and Mg were added to the RNA synthesis assay mixture containing erythroid cell DNA as template, RNA transcription activity was highest, but when Mg was replaced with Mn, denatured calf thymus DNA formed a better template than erythroid cell DNA. In contrast, nucleoplasmic DNA from erythroid cell and liver DNA were the best templates to stimulate RNA transcription when RNA polymerase II and Mn were added to the assay mixture. However, if Mn was replaced with Mg, RNA synthesis activity was drastically reduced when the template was nucleoplasmic DNA of erythroid cell. RNA polymerase I and Mg synthesized GC rich RNA, whereas RNA polymerase II and Mn synthesized AU rich RNA. Sedimentation analysis showed that the molecular weights of the RNA produced by polymerase I were larger when the enzyme was activated with Mg than with Mn, whereas those of the RNA produced by polymerase II were larger with Mn than with Mg. Furthermore, RNA produced by polymerase I and Mg using chr...Continue Reading

References

Sep 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·K H FalchukB L Vallee
Dec 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·T BarrettH J Gould
Apr 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·A W StegglesW F Anderson
Apr 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·D Maryanka, H Gould
Jul 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·R AxelG Felsenfeld
Nov 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·G M ManiatisP A Marks
Mar 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·Z DarzynkiewiczB G Arnason
Mar 28, 1972·Biochemistry·B J Schmeckpeper, K D Smith
Jan 1, 1972·Acta Endocrinologica. Supplementum·P ChambonP Nuret
Dec 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·R S Gilmour, J Paul
Jul 1, 1974·Archives of Biochemistry and Biophysics·J A Nicolette, M Babler
Nov 11, 1971·European Journal of Biochemistry·P H ButterworthC J Chesterton
Feb 1, 1971·Proceedings of the National Academy of Sciences of the United States of America·M E Morris, H Gould
Mar 1, 1966·Journal of Molecular Biology·J Paul, R S Gilmour
Feb 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·I Grummt
Jan 1, 1983·Human Genetics·M F Trendelenburg
Jan 1, 1980·Annual Review of Biochemistry·E O Long, I B Dawid
Nov 1, 1982·Journal of Cellular Physiology·M T ShanderR Weinmann
Nov 28, 1966·Journal of Molecular Biology·H Greenberg, S Penman

❮ Previous
Next ❯

Citations

Jun 24, 2003·Biochemical Pharmacology·Jerome A Roth, Michael D Garrick

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.