PMID: 6166103Mar 1, 1981Paper

Specific staining of sialic acid components on sodium dodecyl sulfate polyacrylamide gel

Stain Technology
C UmezuK Takahashi

Abstract

Specific staining of sialic acid components after sodium dodecyl sulfate polyacrylamide gel electrophoresis can be carried out as follows: 1) extract glycoprotein of erythrocyte membranes or serum by the phenol-saline method, 2) electrophorese the extract on 5% polyacrylamide gel containing 0.1% sodium dodecyl sulfate at constant current, 3) treat the gel with chilled 0.04 M HIO4 for 45 minutes, 4) replace the periodic acid solution with one containing resorcinol 0.6 g, conc. HCl 50 ml, 0.1 M CuSO4 0.5 ml and H2O 50 ml, 5) warm the container in boiling water until blue violet sialic acid bands become clear, 6) replace the staining solution with a mixture of equal parts water and concentrated HCl and observe at once.

References

Jul 1, 1975·The Tohoku Journal of Experimental Medicine·K Sagisaka, C Hamada
Oct 1, 1966·Biochemistry·G F SpringerH Tegtmeyer

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