Specific TaqMan allelic discrimination assay for rs1477196 and rs9939609 single nucleotide polymorphisms of FTO gene demonstrated that there is no association between these SNPs and risk of breast cancer in Iranian women

Advanced Biomedical Research
Mahboobeh MojaverMansoor Salehi

Abstract

Breast cancer (BC), is the most common cancer in women, that is the major cause of cancer-related morbidity and mortality in women. Obesity is considered as a major risk factor for BC that increases both the rate and intensity of the disease. Polymorphisms in FTO gene, a known obesity related gene, is shown to be associated with obesity-related traits as well. The aim of this study was to evaluate the association between previously reported single nucleotide polymorphisms (SNPs) of intron 1of FTO gene, rs1477196 and rs9939609 and risk of BC in a subset of Iranian BC patients. We genotyped 99 cases and 100 controls for the two SNPs of rs9939609 and rs1477196 by TaqMan allelic discrimination assay. For each sample in an allelic discrimination assay, a unique pair of fluorescent dye probe is used. One fluorescent dye probe has a perfect match with the wild type allele and the other fluorescent dye probe is perfectly matched to the mutated allele. Our research has shown that the observed differences between case and control groups in the studied SNPs of FTO gene are not statistically significant (P > 0.05). Our findings suggest that there is no association between rs9939609 and rs1477196 polymorphisms in FTO gene and increase in ri...Continue Reading

Citations

Dec 31, 2015·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Kheirollah YariZohreh Rahimi
Nov 23, 2018·Journal of Cancer Research and Clinical Oncology·JiaLing Chen, Bin Du
Feb 25, 2020·Expert Review of Endocrinology & Metabolism·Maryam GholamalizadehSaeid Doaei
May 14, 2020·Journal of Physiological Anthropology·Mahsa MehrdadMohammad Hassan Eftekhari
Dec 10, 2020·Cell & Bioscience·Yanyan WangSuzhan Zhang
Feb 27, 2021·Journal of Cellular and Molecular Medicine·Saeid DoaeiSeyed Alireza Mosavi Jarrahi
Sep 8, 2021·Journal of Cellular and Molecular Medicine·Maryam GholamalizadehSeyed Alireza Mosavi Jarrahi

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Methods Mentioned

BETA
genotyping
PCRs
PCR

Software Mentioned

SPSS

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