Specific targeting of a plasmodesmal protein affecting cell-to-cell communication.

PLoS Biology
Carole ThomasAndrew J Maule

Abstract

Plasmodesmata provide the cytoplasmic conduits for cell-to-cell communication throughout plant tissues and participate in a diverse set of non-cell-autonomous functions. Despite their central role in growth and development and defence, resolving their modus operandi remains a major challenge in plant biology. Features of protein sequences and/or structure that determine protein targeting to plasmodesmata were previously unknown. We identify here a novel family of plasmodesmata-located proteins (called PDLP1) whose members have the features of type I membrane receptor-like proteins. We focus our studies on the first identified type member (namely At5g43980, or PDLP1a) and show that, following its altered expression, it is effective in modulating cell-to-cell trafficking. PDLP1a is targeted to plasmodesmata via the secretory pathway in a Brefeldin A-sensitive and COPII-dependent manner, and resides at plasmodesmata with its C-terminus in the cytoplasmic domain and its N-terminus in the apoplast. Using a deletion analysis, we show that the single transmembrane domain (TMD) of PDLP1a contains all the information necessary for intracellular targeting of this type I membrane protein to plasmodesmata, such that the TMD can be used to ...Continue Reading

References

Apr 30, 1996·Proceedings of the National Academy of Sciences of the United States of America·T Doyle, D Botstein
Jun 23, 1999·The Plant Journal : for Cell and Molecular Biology·P CzernicN Verbruggen
Jun 2, 2001·The Journal of Biological Chemistry·O GriesbeckR Y Tsien
Sep 11, 2001·The Plant Cell·B A PhillipsonJ Denecke
Nov 13, 2002·Plant Physiology·Andreas NebenführDavid G Robinson
Feb 8, 2003·DNA and Cell Biology·Nigel J Taylor, Claude M Fauquet
May 14, 2004·Plant Physiology·Guo-Wei TianVitaly Citovsky
Jun 29, 2004·Protoplasma·E BayerA J Maule
Oct 12, 2004·Annual Review of Cell and Developmental Biology·Gerd Jurgens
Mar 2, 2005·Nature Reviews. Molecular Cell Biology·Enrique Rodriguez-BoulanAnne Müsch
Mar 8, 2005·Trends in Plant Science·Mansour KarimiPierre Hilson
Sep 27, 2005·Current Opinion in Plant Biology·Tetsuya KurataTakuji Wada
Oct 29, 2005·Journal of Virology·Alexey I ProkhnevskyValerian V Dolja
Jan 31, 2006·The Plant Journal : for Cell and Molecular Biology·Keith W EarleyCraig S Pikaard
Mar 24, 2006·The Plant Journal : for Cell and Molecular Biology·Andrey A ZamyatninEugene I Savenkov
Apr 4, 2006·Proceedings of the National Academy of Sciences of the United States of America·Ji-Young LeePhilip N Benfey
Aug 9, 2006·The Journal of General Virology·Masaaki FujikiRoger N Beachy
Oct 31, 2006·Plant Physiology·Georgia DrakakakiNatasha Raikhel
Feb 3, 2007·The Plant Journal : for Cell and Molecular Biology·Amit LevyBernard L Epel
Aug 30, 2008·Cellular and Molecular Life Sciences : CMLS·A Spang

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Citations

Mar 13, 2014·Journal of Experimental Botany·Yoselin Benitez-Alfonso
Dec 26, 2013·Journal of Experimental Botany·Byung-Kook Ham, William J Lucas
May 4, 2013·Annual Review of Plant Biology·Jan MalinskyWidmar Tanner
Dec 6, 2011·Annual Review of Plant Biology·Tessa M Burch-Smith, Patricia C Zambryski
Oct 8, 2014·Annual Review of Cell and Developmental Biology·Kimberly L GallagherChin-Mei Lee
Oct 7, 2014·Current Opinion in Plant Biology·J Paul Knox, Yoselin Benitez-Alfonso
Jul 11, 2008·Plant Physiology·Fatima AlkhalfiouiFrançoise Montrichard
Aug 20, 2008·Proceedings of the National Academy of Sciences of the United States of America·Stefan HothUlrich Z Hammes
Sep 27, 2008·Journal of Virology·Ruyi XiongXueping Zhou
May 3, 2011·PloS One·Lourdes Fernandez-CalvinoAndrew Maule
Nov 3, 2011·PLoS Pathogens·Khalid AmariChristophe Ritzenthaler
Mar 8, 2013·PloS One·Lisa Zalepa-King, Vitaly Citovsky
Oct 6, 2010·Protoplasma·Christine Faulkner, Andy Maule
Oct 13, 2010·Protoplasma·Jens TilsnerLesley Torrance
Nov 3, 2010·Protoplasma·Tae Kyung HyunJae-Yean Kim
Nov 13, 2010·Protoplasma·Karen Bell, Karl Oparka
Dec 3, 2010·Protoplasma·Annette Niehl, Manfred Heinlein
May 1, 2012·Nature Cell Biology·Felix SpiraRoland Wedlich-Söldner
Jul 12, 2008·Molecular Plant-microbe Interactions : MPMI·Michael M GoodinSteven A Lommel
Dec 15, 2015·Current Opinion in Plant Biology·Sofia OteroYoselin Benitez-Alfonso
Oct 30, 2015·Plant Signaling & Behavior·Philip CarellaRobin K Cameron
Aug 28, 2013·Current Opinion in Plant Biology·Jacob O BrunkardPatricia C Zambryski
Jan 19, 2016·Archives of Virology·Paulus W den HollanderJan W M van Lent
Aug 9, 2011·Current Opinion in Plant Biology·Andrew J MauleChristine Faulkner
Mar 24, 2012·Current Opinion in Virology·Jeanmarie Verchot
Mar 24, 2012·Current Opinion in Virology·Christophe Ritzenthaler
Feb 22, 2011·Trends in Plant Science·Jung-Youn Lee, Hua Lu
Oct 12, 2010·Trends in Plant Science·Sébastien MongrandFrançoise Simon-Plas
Oct 12, 2010·Current Opinion in Plant Biology·Xianfeng Morgan Xu, David Jackson
Sep 15, 2009·Trends in Cell Biology·William J LucasJae-Yean Kim

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Methods Mentioned

BETA
electron microscopy
transgenic
transgenically
confocal microscopy
GTPase
protein folding
PCR
Assay

Software Mentioned

BLAST
Clustal
SignalP
Phylodendron
TMHMM
Graph Prism
GraphPad
Leica LCS

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