Specific targeting of phototoxic haptenated liposomes to a hapten-specific B cell lymphoma

Cellular Immunology
K Avrilionis, J M Boggs

Abstract

A method is reported to eliminate B lymphocytes specific for a haptenated lipid by using the lipid hapten to target a photosensitive drug to them. The photosensitizer eosin was coupled to a phospholipid and incorporated into trinitrophenol (TNP)-bearing small unilamellar vesicles of egg phosphatidylcholine (PC) and cholesterol in order to target the photosensitizer to B lymphoma cells (A20-HL) with TNP-specific membrane IgM receptors in vitro. Exposure of the treated cells to visible light led to an antigen-specific toxic effect indicated by inhibition of cell proliferation. A significantly higher concentration of liposomal eosin was required to inhibit control B cells. These were genetically identical B lymphoma cells (A20-2J) which lack only the DNA for the surface antigen receptor. Furthermore, pretreatment with TNP-conjugated keyhole limpet hemocyanin or anti-IgM antibody abolished the antigen-specific toxic effect, confirming that the TNP-targeted liposomal eosin mediates its effect by binding to the Ig antigen receptors on TNP-specific B cells. Incubation of cells with the TNP-bearing phototoxic liposomes at 4 degrees C instead of 37 degrees C did not alter the antigen-specific targeting effect, suggesting that uptake of ...Continue Reading

Citations

Jun 6, 2000·Advanced Drug Delivery Reviews·M Willis, E Forssen

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