PMID: 9188571Jul 1, 1997Paper

Specific targeting to CD4+ cells of recombinant vesicular stomatitis viruses encoding human immunodeficiency virus envelope proteins

Journal of Virology
J E JohnsonJohn K Rose

Abstract

We generated replication-competent, recombinant vesicular stomatitis viruses (VSVs) expressing the human immunodeficiency virus (HIV) envelope protein or an HIV-VSV chimeric envelope protein in which the cytoplasmic domain of the HIV envelope protein was replaced with that from the VSV glycoprotein (G). These recombinants were generated with HIV type 1 (HIV-1) envelopes from both laboratory and primary isolates of HIV-1. The replication-competent recombinant viruses were stable and expressed the foreign proteins at high levels from extra transcription units in VSV. The foreign proteins were processed appropriately and transported to the cell surface. The incorporation of HIV gp120 into VSV particles was demonstrated biochemically only for the construct expressing the chimeric envelopes containing the VSV G cytoplasmic domain. The incorporation of the chimeric HIV envelope protein into the membrane of the recombinant VSV was also demonstrated by electron microscopy with gold-conjugated antibodies. To determine whether specific infection of CD4-positive cells could be demonstrated for these recombinants, we neutralized VSV infectivity due to VSV glycoprotein with anti-VSV serum. The neutralized recombinants expressing the chimeri...Continue Reading

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Citations

Aug 9, 2013·Current Opinion in HIV and AIDS·Christopher L ParksC Richter King
Nov 11, 2014·Expert Review of Vaccines·Mark A Mogler, Kurt I Kamrud
Oct 17, 2014·Journal of Virology·Drishya KurupMatthias J Schnell
Jan 23, 2016·Journal of Virology·Lindsey R Robinson, Sean P J Whelan
Nov 20, 2019·Virus Evolution·Anouk Willemsen, Mark P Zwart
Mar 31, 1999·Proceedings of the National Academy of Sciences of the United States of America·Y ZhaoW F Anderson
Feb 28, 2002·Journal of Virology·Stéphane BiacchesiMichel Brémont
Oct 17, 2006·Journal of Virology·Alexander BukreyevPeter L Collins
May 4, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Elizabeth A RamsburgJohn K Rose
Sep 16, 2006·Springer Seminars in Immunopathology·David K ClarkeStephen A Udem
Feb 26, 2020·Pathogens·Daniela Ogonczyk MakowskaGuy Boivin
Oct 31, 2008·Journal of Virology·Hrefna Kristin JohannsdottirAri Helenius
Jun 19, 2012·Bioengineered·Hsin-Yu LuHung-Jen Liu
Feb 14, 2002·The Journal of General Virology·Christopher J A Ring
Oct 22, 2002·The Journal of General Virology·Gabriele NeumannYoshihiro Kawaoka
Oct 28, 1998·Proceedings of the National Academy of Sciences of the United States of America·P Palese
Nov 2, 2020·Current Opinion in Virology·Gabrielle Scher, Matthias J Schnell

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