Specific uptake of tritiated serotonin in the adult rat pancreas: evidence for the presence of serotonergic fibers

The American Journal of Anatomy
S B KoevaryE C Azmitia

Abstract

Fragments of adult rat pancreas were incubated in vitro with tritiated serotonin at concentrations from 10(-8) to 10(-7) M. The pancreas exhibited an uptake of serotonin which was saturable, with an uptake constant (Km) of 8.75 x 10(-7) M, and a Vmax of 873 pmoles per gram. Specificity was determined by the addition of fluoxetine or norepinephrine to the reaction mixture, both at 10(-5) M. Fluoxetine significantly reduced the 3H-5HT uptake, whereas norepinephrine was without effect. Metergoline (10(-6) M), a specific 5-HT postsynaptic receptor blocker, similarly had no effect on the serotonin uptake in the pancreas. Radioautography of the fragments following uptake of tritiated serotonin (5 x 10(-8) M) revealed silver- grain aggregates dispersed along blood vessels in the interstitial spaces of the exocrine and endocrine pancreas, areas known to be traversed by nerve fibers. There were no silver- grain aggregates over the exocrine or islet parenchymal cells. These data support the hypothesis that the pancreas is innervated by serotonergic fibers. Further evidence for this hypothesis was provided by a preliminary study demonstrating the presence of tryptophan hydroxylase in pancreatic homogenates. These serotonergic fibers may b...Continue Reading

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Citations

Jul 18, 2002·Pancreatology : Official Journal of the International Association of Pancreatology (IAP) ... [et Al.]·W Y Chey, T Chang
Apr 11, 2012·Pancreatology : Official Journal of the International Association of Pancreatology (IAP) ... [et Al.]·Savio G Barreto, Gino T P Saccone
Jul 25, 2012·Annals of the New York Academy of Sciences·Eduardo RoggeroHugo O Besedovsky
Oct 13, 2006·Current Opinion in Gastroenterology·T M Chang, W Y Chey
Mar 22, 1991·The Journal of Comparative Neurology·A L Kirchgessner, J E Pintar
Jan 22, 1998·The American Journal of Physiology·M T Liu, A L Kirchgessner
Mar 20, 2001·American Journal of Physiology. Gastrointestinal and Liver Physiology·J P LiW Y Chey
Dec 1, 1995·International Journal of Pancreatology : Official Journal of the International Association of Pancreatology·F C BrunicardiD K Andersen

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