PMID: 9428678Jan 15, 1998Paper

Specificity and direction of depolymerization of beta-poly(L-malate) catalysed by polymalatase from Physarum polycephalum--fluorescence labeling at the carboxy-terminus of beta-poly(L-malate)

European Journal of Biochemistry
B Gasslmaier, E Holler

Abstract

Beta-poly(L-malate), a major constituent of nuclei in plasmodia of Physarum polycephalum, is enzymatically degraded to L-malate after secretion into the culture medium. This depolymerization is specifically catalysed by an endogenous polymalatase. The mode of action and the specificity criteria have been investigated by employing various chemical derivatives of beta-poly(L-malate), including substitution at the hydroxy-terminus and carboxy-terminus of the polymer, esterification of the pending alpha-carboxylate, and beta-poly(DL-malate). The results of the investigation were summarized in a specificity model that involved recognition of the hydroxy-terminus and of the alpha-carboxylate as substituents of the asymmetric carbon in the malic acid unit. Depolymerization proceeded from the hydroxy-terminus towards the carboxy-terminus, thereby degrading the polymer to L-malate. When the terminal beta-carboxylate had been amidated with the fluorescent N-(1-naphthyl)ethylenediamine, degradation was normal but was arrested at the level of the terminal beta-carboxy-substituted dimer. It should be possible to employ polymalatase as a tool for the detection of branching and other modifications of beta-poly(L-malate).

Citations

Jun 10, 2011·Applied Microbiology and Biotechnology·Huili ZhangPeilin Cen
Mar 26, 2003·European Journal of Biochemistry·Miachael KarlEggehard Holler
Nov 28, 2000·Journal of Bacteriology·S RenzelA Hildebrandt
Aug 10, 2000·European Journal of Biochemistry·B GasslmaierE Holler
Jul 17, 2007·Journal of Controlled Release : Official Journal of the Controlled Release Society·Manabu FujitaJulia Y Ljubimova
Mar 15, 2016·Applied Microbiology and Biotechnology·Zhe ChiZhen-Ming Chi
Jan 1, 2010·Journal of Nanotechnology·José Portilla-AriasEggehard Holler
Jan 2, 2016·International Journal of Biological Macromolecules·Weifeng CaoYinhua Wan
Dec 4, 2020·Trends in Biochemical Sciences·Jianguo ZhangZhimin Tao

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