PMID: 2790048Oct 2, 1989Paper

Specificity and kinetics of hexose transport in Trypanosoma brucei

Biochimica Et Biophysica Acta
R S EisenthalG Holman

Abstract

Transport of 6-deoxy-D-glucose was studied in Trypanosoma brucei in order to characterise the kinetics of hexose transport in this organism using a nonphosphorylated sugar. Kinetic parameters for efflux and entry, measured using zero-trans and equilibrium exchange protocols, indicate that the transporter is probably kinetically symmetrical. Comparison of the kinetic constants of D-glucose metabolism with those for 6-deoxy-D-glucose transport shows that transport across the plasma membrane is likely to be the rate-limiting step of glucose utilisation. The transport rate is nevertheless very fast and 6-deoxy-D-glucose, at concentrations below Km, enters the cells with a half filling time of less than 2 s at 20 degrees C. Thus the high metabolic capacity of these organisms is matched by a high transport rate. The structural requirements for the trypanosome hexose transporter were explored by measuring inhibition constants (Ki) for a range of D-glucose analogues including fluoro and deoxy sugars as well as epimeric hexoses. The relative affinities shown by these analogues indicated H-bonds from the carrier to the C-3, C-4 and C-5 hydroxyl oxygens and from the C-1 and C-3 hydroxyl hydrogens to the binding site. Hydrophobic interacti...Continue Reading

References

Aug 2, 1984·Nature·F A Quiocho, N K Vyas
Jan 1, 1983·The International Journal of Biochemistry·J K Kiaira, R M Njogu

❮ Previous
Next ❯

Citations

Oct 1, 1995·Journal of Bioenergetics and Biomembranes·B M BakkerP A Michels
Sep 1, 1990·Molecular and Biochemical Parasitology·M Parsons, B Nielsen
Jul 1, 1991·Molecular and Biochemical Parasitology·T Munoz-AntoniaE Ullu
May 1, 1992·Molecular and Biochemical Parasitology·F Bringaud, T Baltz
Nov 1, 1992·Molecular and Biochemical Parasitology·L A KnodlerM R Edwards
Jul 1, 1993·Molecular and Biochemical Parasitology·A J FryR Eisenthal
Dec 1, 1995·Molecular and Biochemical Parasitology·H R HotzC Clayton
Mar 24, 2004·Biochemical Pharmacology·Laurent AzemaMichael P Barrett
May 4, 2001·International Journal for Parasitology·F R Opperdoes, P A Michels
Feb 1, 1997·Molecular and Biochemical Parasitology·I D GoodyerR Eisenthal
May 9, 1998·Molecular and Biochemical Parasitology·M P BarrettT Baltz
Jan 1, 1994·Parasitology·C K LangfordS M Landfear
Aug 11, 2006·Parasitology·L C MilettiB U Stambuk
May 2, 2002·Proceedings of the National Academy of Sciences of the United States of America·Janine R RoperMichael A J Ferguson
Aug 16, 1994·Proceedings of the National Academy of Sciences of the United States of America·E TetaudT Baltz
Sep 1, 1999·Proceedings of the National Academy of Sciences of the United States of America·B M BakkerH V Westerhoff
Jun 1, 1993·European Journal of Biochemistry·A Seyfang, M Duszenko
Apr 1, 1996·European Journal of Biochemistry·J N WaitumbiT Baltz
Mar 2, 2012·PLoS Computational Biology·Fiona AchcarRainer Breitling
Sep 12, 2006·Bioorganic & Medicinal Chemistry Letters·Michael D UrbaniakMichael A J Ferguson
Aug 3, 1994·Biochimica Et Biophysica Acta·B H ter Kuile, M Cook
Sep 3, 2003·Helicobacter·George L Mendz, Brendan P Burns
Feb 20, 2007·Molecular and Biochemical Parasitology·Dayana Rodriguez-ContrerasScott M Landfear
Dec 1, 2006·Advances in Parasitology·Michael P Barrett, Ian H Gilbert
Oct 29, 2000·Journal of Applied Microbiology·M Hasne, M P Barrett
May 18, 1999·The Journal of Biological Chemistry·B M BakkerH V Westerhoff
Jun 16, 2005·The Journal of Biological Chemistry·Marie-Astrid AlbertPaul A M Michels
Mar 24, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Mohamed A O AbdelfattahMansour Sobeh
May 9, 1998·Molecular and Biochemical Parasitology·E Pays, D P Nolan
Dec 30, 1998·Trends in Biochemical Sciences·A R WalmsleyG W Gould
Sep 28, 1998·Biochimica Et Biophysica Acta·L L Vieira

❮ Previous
Next ❯

Related Concepts

Related Feeds

African Trypanosomiasis

African trypanosomiasis, also known as sleeping sickness, is an insect-borne parasitic disease of humans and other animals. It is caused by protozoa of the species Trypanosoma brucei and almost invariably progresses to death unless treated. Discover the latest research on African trypanosomiasis here.