PMID: 40209Jul 11, 1979

Specificity and mode of cleavage of the pH 4.0 endonuclease from adenovirus type 2 - infected KB cells

Nucleic Acids Research
R PadmanabhanW Doerfler

Abstract

Adenovirus type 2 or lambda DNA was digested with the pH 4.0 endonuclease, purified from adenovirus 2-infected KB cells. The enzyme produces a limit digest of approximate size in the range of 140-210 base pairs long. The termini of the DNA fragments generated by the endonuclease digestion had 3'-P and 5'-OH groups. The 3' and 5' end groups of the products were analyzed. Our data indicate that 3' end group was a purine (68-76%), dA occuring about twice the frequency of dG. The 5' end group was either dG or dC with equal frequency. Data obtained by treatment of the 5' labeled endonuclease product of lambda DNA with single-strand specific S1 nuclease from Asperigillus oryzae or exonuclease VII from Escherichia coli indicated that the majority of the products had a short 5' protruding ends. The mode of cleavage of this endonuclease seems to be through initial formation of several single-strand breaks with some base specificity. If these breaks are at close proximity on opposite strands, double-stranded fragments with protruding ends are generated.

References

Dec 11, 1981·Nucleic Acids Research·G D FrenkelN Berns

Citations

Mar 1, 1977·European Journal of Biochemistry·U ReifW Doerfler
Mar 1, 1977·European Journal of Biochemistry·U ReifW Doerfler
Jul 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·W G BurtonR Sager
Apr 15, 1977·European Journal of Biochemistry·C Cajean-FeroldiJ Chantepie-Auray
Jan 1, 1967·Proceedings of the National Academy of Sciences of the United States of America·R Wu, A D Kaiser
Oct 15, 1973·Biochemical and Biophysical Research Communications·M MéchaliM Girard
Aug 28, 1971·Journal of Molecular Biology·B T BurlinghamL Philipson
Feb 14, 1968·Journal of Molecular Biology·M E Gottesman, M B Yarmolinsky

Related Concepts

Endonuclease
DNA, Viral
Alkaline Phosphatase
Alkaline DNase
Substrate Specificity
EXO1
Hydrogen-Ion Concentration
Adenovirus Infections
Adenoviruses, Human

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