Specificity and prognostic validation of a polyclonal antibody to detect Six1 homeoprotein in ovarian cancer.

Gynecologic Oncology
Lubna QamarKian Behbakht

Abstract

The presence of Six1 mRNA gene portends a poor prognosis in ovarian cancer. We describe validation of a Six1 specific antibody and evaluate its association with tumorigenicity and prognosis in ovarian cancer. A Six1 antibody (Six1cTerm) was raised to residues downstream of the Six1 homeodomain, representing its unique C-terminus as compared to other Six family members. Cells were transfected with Six1-Six6 and Western blot was performed to demonstrate Six1 specificity. Ovarian cancer cell lines were analyzed for Six1 mRNA and Six1cTerm and tumorigenicity was evaluated. Ovarian cancer tissue microarrays (OTMA) were analyzed for Six1cTerm by immunohistochemistry and scored by two blinded observers. The metastatic tumors of 15 stage IIIC high grade serous ovarian cancers were analyzed with Six1 mRNA and Six1cTerm and expression was compared to clinical factors and survival. The Six1cTerm antibody is specific for Six1. Cell line tumorigenicity in SCID mice correlates with Six1 levels both by mRNA(p=0.001, Mann-Whitney U test) and by protein (presence vs. absence, p=0.05 Fischer's Exact test). Six1 protein was present in up to 54% of OTMA specimens. Six1 protein expression in omental/peritoneal metastases correlated with worsened su...Continue Reading

References

Nov 1, 1981·The Journal of Clinical Investigation·R C BastR C Knapp
Sep 27, 1994·Proceedings of the National Academy of Sciences of the United States of America·K OrthJ Sambrook
Oct 29, 1997·Proceedings of the National Academy of Sciences of the United States of America·P X XuR L Maas
Oct 15, 1998·Proceedings of the National Academy of Sciences of the United States of America·H L FordA B Pardee
Feb 19, 2000·Proceedings of the National Academy of Sciences of the United States of America·M KurusuK Furukubo-Tokunaga
May 10, 2000·The Journal of Biological Chemistry·H L FordD C Seldin
May 24, 2000·Journal of Clinical Pathology·C WinchesterM Thomas
Jul 6, 2000·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·K KawakamiK Ikeda
Oct 3, 2002·Nature Reviews. Cancer·Cory Abate-Shen
Oct 31, 2002·Development·Jianwu Bai, Denise Montell
Jun 5, 2003·Developmental Biology·James J FabrizioStephen DiNardo
Jul 23, 2003·Development·Weiming ZhengPin-Xian Xu
Dec 26, 2003·Development·Hidenori OzakiKiyoshi Kawakami
May 5, 2004·Proceedings of the National Academy of Sciences of the United States of America·Ricardo D ColettaHeide L Ford
Apr 5, 2005·Cancer Research·Kelly J ReichenbergerHeide L Ford
Sep 30, 2006·British Journal of Cancer·K T NgS-T Fan
Apr 10, 2008·International Journal of Cancer. Journal International Du Cancer·Fang WanLucia Pirisi
Jul 29, 2008·International Journal of Cancer. Journal International Du Cancer·Wei HePhilip S Low
Dec 6, 2008·Advances in Cancer Research·Kimberly L ChristensenHeide L Ford
May 8, 2009·Genesis : the Journal of Genetics and Development·Brandon P Weasner, Justin P Kumar
Jan 16, 2010·BMC Developmental Biology·Ricardo D ColettaHeide L Ford
May 5, 2010·Gynecologic Oncology·C Gómez-RaposoA Redondo
Nov 26, 2010·Cancer Cell International·Thangarajan RajkumarSeshadri A Ramakrishnan

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Citations

Oct 12, 2013·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Liu HuaWang Xiaojian
Dec 5, 2012·BMC Medical Genomics·Ahmed SadequeSandra L Rodriguez-Zas
Mar 7, 2014·The Biochemical Journal·Hui ZhaoLu Gao
May 29, 2014·Experimental and Molecular Pathology·Huixin LvZhenhua Lin
Oct 26, 2016·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Juan YangKeqiang Zhang
Jan 25, 2013·Nature Reviews. Cancer·Bram De Craene, Geert Berx
Feb 6, 2017·Brain Structure & Function·Stefan Washausen, Wolfgang Knabe
Sep 10, 2017·The International Journal of Biochemistry & Cell Biology·Hengbo ZhouRui Zhao
Mar 19, 2014·Journal of Cell Science·Jenean H O'BrienHeide L Ford
May 29, 2018·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Chao YuXiao-Rui Yu

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