Specificity and symmetry in the interaction of calmodulin domains with the skeletal muscle myosin light chain kinase target sequence.

The Journal of Biological Chemistry
A BarthP M Bayley

Abstract

The specificity of interaction of the isolated N- and C-terminal domains of calmodulin with peptide WFFp (Ac-KRRWKKNFIAVSAANRFK-amide) and variants of the target sequence of skeletal muscle myosin light chain kinase was investigated using CD and fluorescence. Titrations show that two molecules of either domain bind to 18-residue target peptides. For WFFp, the C-domain binds with 4-fold higher affinity to the native compared with the non-native site; the N-domain shows similar affinity for either site. The selectivity of the C-domain suggests that it promotes occupancy of the correct binding site for intact calmodulin on the target sequence. Far UV CD spectra show the extra helicity induced in forming the 2:1 C-domain-peptide or the 1:1:1 C-domain-N-domain-peptide complex is similar to that induced by calmodulin itself; binding of the C-domain to the Trp-4 site is essential for developing the full helicity. Calmodulin-MLCK-peptide complexes show an approximate two-fold rotational relationship between the two highly homologous domains, and the 2:1 C (or N)-domain-peptide complexes evidently have a similar rotational symmetry. This implies that a given domain can bind sequences with opposite peptide polarities, significantly incre...Continue Reading

References

Aug 1, 1992·Journal of Biochemistry·M Itakura, T Iio
Jan 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·A PersechiniT N Davis
Feb 1, 1990·Trends in Biochemical Sciences·K T O'Neil, W F DeGrado
Nov 1, 1989·Analytical Biochemistry·S C Gill, P H von Hippel
Feb 13, 1987·Biochemical and Biophysical Research Communications·S KlumppJ E Schultz
Jun 30, 1985·Biochimica Et Biophysica Acta·D NewtonP Cohen
Apr 1, 1986·Archives of Biochemistry and Biophysics·R F SteinerD Needleman
Jul 20, 1981·FEBS Letters·J KuznickiP Cohen
Oct 1, 1984·Journal of Biochemistry·O Minowa, K Yagi
Jan 1, 1982·Advances in Protein Chemistry·C B Klee, T C Vanaman
Nov 7, 1995·Proceedings of the National Academy of Sciences of the United States of America·A Houdusse, C Cohen
Sep 1, 1995·Nature Structural Biology·W J Chazin
Sep 1, 1995·Nature Structural Biology·H KuboniwaA Bax
Jan 1, 1995·Annual Review of Biophysics and Biomolecular Structure·A Crivici, M Ikura
Jan 1, 1995·Trends in Biochemical Sciences·P JamesE Carafoli
Nov 1, 1995·Protein Science : a Publication of the Protein Society·W A FindlayP M Bayley
May 1, 1996·The Biochemical Journal·M V MedvedevaN B Gusev
Jul 1, 1996·Protein Science : a Publication of the Protein Society·P M BayleyS R Martin

❮ Previous
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Citations

Jul 18, 2009·Journal of Computational Neuroscience·Michael J ByrneYoshihisa Kubota
May 9, 2001·Biochimica Et Biophysica Acta·M V MedvedevaN B Gusev
May 18, 2013·Accounts of Chemical Research·Shiroh FutakiKoji Asami
Feb 5, 2009·Proceedings of the National Academy of Sciences of the United States of America·Swarnendu Tripathi, John J Portman
Dec 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·H CharbonneauJ A Beavo
May 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·P BrandtT C Vanaman
Aug 16, 2000·Proceedings of the National Academy of Sciences of the United States of America·S QuetglasM Seagar
Jan 21, 2010·The Journal of Biological Chemistry·Yubin ZhouJenny J Yang
Nov 21, 2002·Protein Science : a Publication of the Protein Society·Stephen R Martin, Peter M Bayley
Dec 14, 1999·Protein Science : a Publication of the Protein Society·S R MartinP M Bayley
Sep 7, 2000·Protein Science : a Publication of the Protein Society·L MasinoP M Bayley
Feb 5, 2002·Annual Review of Physiology·Yoshiro Saimi, Ching Kung
Jan 15, 1999·Acta Physiologica Scandinavica·J T StullG Zhi
Mar 26, 2013·Protein Science : a Publication of the Protein Society·Nicholas J Anthis, G Marius Clore
Jun 5, 2004·FEBS Letters·Stephen R Martin, Peter M Bayley
May 15, 2009·Chembiochem : a European Journal of Chemical Biology·Ping YanM Uljana Mayer
Sep 19, 2003·Biochemical and Biophysical Research Communications·Narasimhaiah SitaramRamakrishnan Nagaraj

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