PMID: 9422095Jan 9, 1998Paper

Specificity clusters of MUC1-reactive mouse monoclonal antibodies

Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine
F G Hanisch

Abstract

The ISOBM TD-4 Workshop antibodies 122-177 were grouped according to their reactivity with: (a) monomeric MUC1 peptide (TAP25); (b) the pentameric tandem repeat peptide (TR-5), both unglycosylated or as their GalNAc-substituted derivatives, and (c) the lactation or tumor-associated glycoforms of MUC1. The antibodies were grouped into nine clusters: Clusters 1-4 comprise those antibodies that bind to MUC1 tandem repeat peptide (monomeric) and define sequential epitopes differentially affected by glycosylation (GalNAc substitution) in the peptide motifs VTSA and GSTA. The ISOBM-163 antibody in cluster 5 defines Tn antigen as a site-specific glycotope within the tandem repeat peptide. The antibodies in clusters 6 and 7A recognize peptide epitopes within the tandem repeats, although these seem to be conformationally dependent on complex glycosylation or on oligomeric peptide repetition. Antibodies in clusters 7B bind to a conformational epitope not related to the tandem repeat peptide or to a carbohydrate epitope (cluster 7BC). Antibodies in cluster 8 showed a high selective binding to tumor-associated epitopes on MUC1 from mammary carcinoma cells. Cluster 9 antibodies were not reactive on any of the tested antigens and may be argu...Continue Reading

Citations

Feb 13, 2002·Hybridoma and Hybridomics·W QiR Madiyalakan
Oct 4, 2007·Journal of Pineal Research·Analía M FurioD P Cardinali
Jul 5, 2006·The FEBS Journal·S R Pandi-PerumalR Hardeland
Oct 14, 2003·Pathology·Chooi-Fun LeongHussin Noor Hamidah
Aug 5, 2000·International Journal of Cancer. Journal International Du Cancer·R J Quin, M A McGuckin
May 6, 2008·British Journal of Cancer·J CreaneyM J Millward
Jan 25, 2011·Angewandte Chemie·Tongzhong JuRichard D Cummings
Jun 22, 1999·The Journal of Biological Chemistry·S MüllerF G Hanisch

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antibody Specificity

Antibodies produced by B cells are highly specific for antigen as a result of random gene recombination and somatic hypermutation and affinity maturation. As the main effector of the humoral immune system, antibodies can neutralize foreign cells. Find the latest research on antibody specificity here.

Related Papers

Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine
C A ReisHenrik Clausen
Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine
K O Lloyd, V Kudryashov
Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine
M ReddishB M Longenecker
Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine
P CiborowskiO J Finn
Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine
D ScholJ Hilgers
© 2021 Meta ULC. All rights reserved