PMID: 4904641Sep 1, 1969Paper

Specificity in the assembly of multisubunit proteins

Proceedings of the National Academy of Sciences of the United States of America
R A Cook, D E Koshland

Abstract

The recoveries of activity in the presence of mixtures of several enzymes and a cellular debris were compared with the recoveries of the pure enzymes. In the acid-dissociation experiments, no interference from foreign proteins was observed nor were any cross hybrids between subunits of different enzymes found. This suggests that the intersubunit binding sites are highly specific and have been selected over evolutionary time for correct assembly. In the urea experiments, cross hybridization and decreased yields were observed in a few cases but in most cases the "foreign" unfolded chains did not influence the recovery of the test enzyme. The results suggest that compartmentalization, temporal or spatial, will not be required as far as assembly of subunits of cytoplasmic enzymes is concerned. The results suggest some cross reaction might occur if all peptide chains were unfolding together. If folding occurs during or immediately after translation, this difficulty would be avoided.

References

Nov 1, 1967·Biochemistry·J A Reynolds, M J Schlesinger
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Citations

Nov 1, 1978·Die Naturwissenschaften·R Jaenicke
Jan 1, 1982·Biophysics of Structure and Mechanism·R Jaenicke
Jan 1, 1976·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·R C Ruth, F Wold
Oct 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·G G BorisyR A Klugman
Feb 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·J Hamlin, I Zabin
Feb 1, 1971·European Journal of Biochemistry·U Lindberg, S Eriksson
Jan 7, 2005·The FEBS Journal·Giampiero MeiAlessandro Finazzi-Agrò
Oct 18, 1973·Biochimica Et Biophysica Acta·J TorresaniS Lissitzky
Jan 1, 1987·Progress in Biophysics and Molecular Biology·R Jaenicke
Sep 14, 1976·Biochimica Et Biophysica Acta·H Tenenbaum-Bayer, A Levitzki
Nov 1, 1972·The Biochemical Journal·H G Nimmo, K F Tipton
Oct 21, 1985·Journal of Theoretical Biology·D de Vienne, F Rodolphe
Oct 1, 1972·Archives of Biochemistry and Biophysics·M Kochman, D Kwiatkowska
Dec 29, 1978·Journal of Molecular Evolution·G E Collier, R J MacIntyre

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