Specificity of 3D MSC Spheroids Microenvironment: Impact on MSC Behavior and Properties.

Stem Cell Reviews and Reports
Aleksandra JaukovićMilena Mourdjeva

Abstract

Mesenchymal stem cells (MSC) have been considered the promising candidates for the regenerative and personalized medicine due to their self-renewal potential, multilineage differentiation and immunomodulatory capacity. Although these properties have encouraged profound MSC studies in recent years, the majority of research has been based on standard 2D culture utilization. The opportunity to resemble in vivo characteristics of cells native niche has been provided by implementation of 3D culturing models such as MSC spheroid formation assesed through cells self-assembling. In this review, we address the current literature on physical and biochemical features of 3D MSC spheroid microenvironment and their impact on MSC properties and behaviors. Starting with the reduction in the cells' dimensions and volume due to the changes in adhesion molecules expression and cytoskeletal proteins rearrangement resembling native conditions, through the microenvironment shifts in oxygen, nutrients and metabolites gradients and demands, we focus on distinctive and beneficial features of MSC in spheroids compared to cells cultured in 2D conditions. By summarizing the data for 3D MSC spheroids regarding cell survival, pluripotency, differentiation, ...Continue Reading

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Citations

Apr 4, 2021·Cells·Agnieszka KaminskaAnna Sarnowska
Apr 21, 2021·Stem Cell Reviews and Reports·Kejia LiZigang Ge
Aug 4, 2021·Journal of Cardiovascular Translational Research·Olivier SchusslerYves Lecarpentier
Apr 27, 2021·Frontiers in Cell and Developmental Biology·Anastasiya A GorkunIrina N Saburina

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Methods Mentioned

BETA
bioprinting
flow
confocal microscopy
histone acetylation
acetylation
PCR

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