PMID: 6162061Jan 24, 1981Paper

Specificity of an immunoprecipitin test for non-A non-B hepatitis

Lancet
D J SuhR Williams

Abstract

An immunodiffusion system detecting an antigen showing immunological identity with international laboratory reference sera was developed by use of acute-phase and recovery sera from patients with transmission-proven non-A, non-B acute hepatitis. In other liver diseases the antigen was also present in a high proportion of patients and there were similar findings in conditions with high levels of circulating immune complexes in the serum. Fractionation of antigen-containing sera by column chromatography, polyethylene glycol treatment, and reduction and alkylation also suggested that immune complexes may be responsible for antigenic activity. The precipitation lines did not develop in the presence of 10 mmol/l EDTA or at a pH less than 8.0 and the reactant in "antibody" containing sera was shown not to be an immunoglobulin. Although the transmission of non-A, non-B hepatitis by blood and blood products shows that viral antigens are likely to be present in the circulation, this study shows that immune reactions apparently closely associated with the infection may be detecting immune complexes rather than specific viral components and emphasises the need for careful evaluation of the specificity of other reported serological tests f...Continue Reading

Citations

Jul 11, 1981·Lancet·A Compston
Jul 19, 1984·The New England Journal of Medicine·S M Feinstone, J H Hoofnagle
May 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·K H BurkG R Dreesman
May 1, 1990·Journal of Gastroenterology and Hepatology·H GuptaB N Tandon
Jun 1, 1985·The American Journal of the Medical Sciences·J Shorey
Jan 1, 1981·Journal of Medical Virology·J H Hoofnagle
Jan 1, 1983·Journal of Medical Virology·H OhoriN Ishida
Apr 1, 1985·Journal of Medical Virology·J TohmatsuT Shikata
Mar 1, 1985·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·H ShiraishiR H Purcell
Sep 1, 1981·Liver·J H Hoofnagle, S M Feinstone
Sep 1, 1982·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·H P DienesH Lobeck
Apr 1, 1982·Gastroenterologia Japonica·A E GimsonR Williams

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