PMID: 7018577May 29, 1981Paper

Specificity of chromatin transcription in vitro. Asymmetric transcription of the globin gene by Escherichia coli RNA polymerase

Biochimica Et Biophysica Acta
E Pays, R S Gilmour

Abstract

The transcription of globin genes in mouse foetal liver chromatin and nuclei by exogenous Escherichia coli RNA polymerase is prone to artifacts due to RNA-dependent transcription of endogenous mRNA sequences. This is particularly evident when Mn2+ is used as divalent cation in the RNA transcription reaction. However, substitution or supplementation with Mg2+ eliminates this artifact and gives essentially asymmetric DNA-dependent transcription by the polymerase. In this paper we discuss a number of general criteria which can be applied to test the validity of specific gene transcription in vitro.

References

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Apr 11, 1977·Biochemical and Biophysical Research Communications·M Zasloff, G Felsenfeld
Jan 1, 1979·Nucleic Acids Research·G F CrouseP Doty
Mar 28, 1979·Biochimica Et Biophysica Acta·E PaysR S Gilmour
Jun 1, 1978·Nucleic Acids Research·C Van Broeckhoven, R De Wachter
Sep 3, 1976·Science·H Weintraub, M Groudine
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Aug 28, 1973·Biochemistry·W F MarzluffR C Huang

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Citations

Jul 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·R S GilmourJ Paul
Mar 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·D K Trask, M T Muller
Feb 1, 1985·International Journal of Radiation Biology and Related Studies in Physics, Chemistry, and Medicine·R S NairnJ M Clarkson
Apr 15, 1983·Experimental Cell Research·D W HarrisT J Beebee
May 1, 1985·Bioscience Reports·J D Zhu, J Paul

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